MARC details
| 000 -LEADER |
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| fixed length control field |
02965nam a22003377a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
240723s20242024 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
2701-0198 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
38419396 |
| 245 ## - TITLE STATEMENT |
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| Title |
Exploring the Impact of TLR-2 Signaling on miRNA Dysregulation in Intervertebral Disc Degeneration. |
| 251 ## - Source |
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| Source |
Advances in Biology Online. :e2300581, 2024 Feb 28 |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
adv. biol. :e2300581, 2024 Feb 28 |
| 253 ## - Journal Name |
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| Journal name |
Advanced biology |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2024 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Publication date |
2024 Feb 28 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
aheadofprint |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Medline status |
Publisher |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2024-07-23 |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
Toll-like receptors (TLRs) are key mediators of inflammation in intervertebral disc (IVD) degeneration. TLR-2 activation contributes to the degenerative process by increasing the expression of extracellular matrix-degrading enzymes, pro-inflammatory cytokines, and neurotrophins. As potent post-transcriptional regulators, microRNAs can modulate intracellular mechanisms, and their dysregulation is known to contribute to numerous pathologies. This study aims to investigate the impact of TLR-2 signaling on miRNA dysregulation in the context of IVD degeneration. Small-RNA sequencing of degenerated IVD cells shows the dysregulation of ten miRNAs following TLR-2 activation by PAM2CSK4. The miR-155-5p is most significantly upregulated in degenerated and non-degenerated annulus fibrosus and nucleus pulposus cells. Sequence-based target and pathway prediction shows the involvement of miR-155-5p in inflammation- and cell fate-related pathways and TLR-2-induced miR-155-5p expression leads to the downregulation of its target c-FOS. Furthermore, changes specific to the activation of TLR-2 through fragmented fibronectin are seen in miR-484 and miR-487. Lastly, miR-100-3p, miR-320b, and miR-181a-3p expression exhibit degeneration-dependent changes. These results show that TLR-2 signaling leads to the dysregulation of miRNAs in IVD cells as well as their possible downstream effects on inflammation and degeneration. The identified miRNAs provide important opportunities as potential therapeutic targets for IVD degeneration and low back pain. Copyright © 2024 The Authors. Advanced Biology published by Wiley-VCH GmbH. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Indexing |
Automated |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Orthopedic Surgery |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Mesfin, Addisu |
| Institution Code |
MWHC |
| 790 ## - Authors |
|---|
| All authors |
Cazzanelli P, Lamoca M, Hausmann ON, Mesfin A, Puvanesarajah V, Hitzl W, Haglund L, Wuertz-Kozak K |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1002/adbi.202300581">https://dx.doi.org/10.1002/adbi.202300581</a> |
| Public note |
https://dx.doi.org/10.1002/adbi.202300581 |
| 858 ## - ORCID |
|---|
| ORCID text |
Cazzanelli, Petra |
| Orcid |
<a href="https://orcid.org/0009-0007-3114-951XI - Wuertz-Kozak, Karin">https://orcid.org/0009-0007-3114-951XI - Wuertz-Kozak, Karin</a> |
| -- |
<a href="https://orcid.org/0000-0002-3281-4629">https://orcid.org/0000-0002-3281-4629</a> |
| Name |
https://orcid.org/0009-0007-3114-951XI - Wuertz-Kozak, Karin |
| -- |
https://orcid.org/0000-0002-3281-4629 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Journal Article |
| Item type description |
Article |
