MARC details
| 000 -LEADER |
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| fixed length control field |
03786nam a22005297a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
160523s20152015 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1559-047X |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
25051518 |
| 245 ## - TITLE STATEMENT |
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| Title |
Biphasic presence of fibrocytes in a porcine hypertrophic scar model. |
| 251 ## - Source |
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| Source |
Journal of Burn Care & Research. 36(3):e125-35, 2015 May-Jun. |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
J Burn Care Res. 36(3):e125-35, 2015 May-Jun. |
| 253 ## - Journal Name |
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| Journal name |
Journal of burn care & research : official publication of the American Burn Association |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2015 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2015 |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2016-05-24 |
| 501 ## - WITH NOTE |
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| Local holdings |
Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
The duroc pig has been described as a promising animal model for use in the study of human wound healing and scar formation. However, little is known about the presence and chronology of the fibrocyte cell population in the healing process of these animals. Wounds known to form scar were created on red duroc swine (3" x 3") with a dermatome to a total depth of either 0.06 inches or 0.09 inches. These wounds were allowed to heal completely and biopsies were done at scheduled time points during the healing process. Biopsies were formalin fixed and paraffin embedded for immunohistochemical analysis. Porcine reactive antibodies to CD-45 and procollagen-1 and a human reactive antibody to LSP-1 were used to detect the presence of fibrocytes in immunohistochemistry, an immunocytochemistry. Initial immunohistochemical studies showed evidence of a biphasic presence of fibrocytes. Pigs with 0.06 inches deep wounds showed positive staining for CD-45 and LSP-1 within highly cellular areas at days 2 and 4 after wounding. Additional animals with 0.09 inches deep wounds showed positive staining within similar areas at days 56, 70, and 113 after wounding. There was no immunohistochemical evidence of fibrocytes in skin biopsies taken at days 14, 28, or 42. Procollagen-1 staining was diffused in all samples. Cultured cells were stained for CD-45, LSP-1, and procollagen-1 by immunocytochemistry. These data confirm that fibrocytes are indeed present in this porcine model. We conclude that these cells are present after initial wounding and later during scar formation and remodeling. We believe that this is an evidence of a biphasic presence of fibrocytes, first as an acute response to skin wounding followed by later involvement in the remodeling process, prompted by continued inflammation in a deep partial thickness wound. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Burns/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Cicatrix, Hypertrophic/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Fibroblasts/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Skin/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Animals |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Antigens, CD34/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Burns/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Cicatrix, Hypertrophic/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Disease Models, Animal |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Fibroblasts/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Skin/in [Injuries] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Swine |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Wound Healing/ph [Physiology] |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Surgery/Burn Services |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Research Support, N.I.H., Extramural |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Jordan, Marion H |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Mauskar, Neil A |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Mino, Matthew J |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Moffatt, Lauren T |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Prindeze, Nicholas J |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Shupp, Jeffrey W |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Travis, Taryn E |
| 790 ## - Authors |
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| All authors |
Ghassemi P, Jordan MH, Mauskar NA, Mino MJ, Moffatt LT, Prindeze NJ, Ramella-Roman JC, Shupp JW, Travis TE |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="http://dx.doi.org/10.1097/BCR.0000000000000097">http://dx.doi.org/10.1097/BCR.0000000000000097</a> |
| Public note |
http://dx.doi.org/10.1097/BCR.0000000000000097 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Journal Article |
| Item type description |
Article |
