MARC details
| 000 -LEADER |
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| fixed length control field |
04177nam a22004817a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
171205s20172017 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1527-7941 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
29140837 |
| 245 ## - TITLE STATEMENT |
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| Title |
Disruption of Biofilms and Neutralization of Bacteria Using Hypochlorous Acid Solution: An In Vivo and In Vitro Evaluation. |
| 251 ## - Source |
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| Source |
Advances in Skin & Wound Care. 30(12):543-551, 2017 Dec |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
Adv Skin Wound Care. 30(12):543-551, 2017 Dec |
| 253 ## - Journal Name |
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| Journal name |
Advances in skin & wound care |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2017 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2018 |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2017-12-05 |
| 501 ## - WITH NOTE |
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| Local holdings |
Available online from MWHC library: 2001 - present |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
CONCLUSIONS: These findings support the use of VWS in the treatment of wounds with biofilms and to reduce the bioburden of venous stasis ulcers. While VWS-treated biofilms had higher biomass than CWS- and saline-treated biofilms, most of the cellular component was not viable. Ultimately, VWS had a similar effectiveness to CWS in eliminating bacteria but with lower cytotoxicity. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
METHODS: Plates coated with collagen films incubated with active bacteria cultures to yield biofilm mimics were treated with VWS, 1% and 10% povidone-iodine (PI), 0.05% chlorhexidine wound solution (CWS), or normal saline for 3 or 10 minutes. Biofilms were then analyzed for biomass density using a crystal violet assay, quantitative cultures, and fluorescent microscopy. Cytotoxicity was measured using neutral red uptake by primary human dermal fibroblasts. Pre- and postcleansing exudates and swab samples obtained from venous stasis wounds of patients were processed and plated on a series of selective agar plates for bacteria typing and quantification. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
OBJECTIVES: The aims of this study were to assess the effectiveness of a hypochlorous acid-based wound cleanser (Vashe Wound Solution [VWS], SteadMed Medical, Fort Worth, Texas) in disrupting methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms relative to other cleansers using an in vitro collagen biofilm model and to evaluate cleansers' cytotoxicity. The bioburden reduction of venous stasis wounds by VWS and another cleanser was evaluated. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
RESULTS: All agents tested significantly neutralized methicillin-resistant S aureus and P aeruginosa biofilms compared with saline control as assessed by crystal violet assay and fluorescent microscopy assays. Undiluted VWS was significantly less cytotoxic compared with 1% PI, CWS, and 10% PI (in increasing order of cytotoxicity). There was no significant difference in bacterial reduction in wounds after treatment with VWS or CWS for any type of bacteria examined using selective media. In wounds that were treated with VWS or CWS, there was a similar percentage reduction in bacterial colony-forming units from precleansing levels when plated on tryptic soy agar, MacConkey, streptococcal, and mannitol salt agar plates. Plates treated with CWS trended toward higher bacterial reduction on nonselective and gram-negative agars, whereas VWS trended toward higher bacterial reduction in Streptococcus-selective agars. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Anti-Bacterial Agents/pd [Pharmacology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Anti-Infective Agents, Local/pd [Pharmacology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Biofilms/de [Drug Effects] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Hypochlorous Acid/pd [Pharmacology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Methicillin-Resistant Staphylococcus aureus/de [Drug Effects] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Varicose Ulcer/mi [Microbiology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Humans |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Pseudomonas aeruginosa/de [Drug Effects] |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Health Research Institute |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Firefighters' Burn and Surgical Research Laboratory |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Alkhalil, Abdulnaser |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Carney, Bonnie C |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Day, Anna |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Hoffman, Hilary N |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Moffatt, Lauren T |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Shupp, Jeffrey W |
| 790 ## - Authors |
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| All authors |
Alkhalil A, Carney BC, Day A, Hoffman HN, Moffatt LT, Shupp JW |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1097/01.ASW.0000526607.80113.66">https://dx.doi.org/10.1097/01.ASW.0000526607.80113.66</a> |
| Public note |
https://dx.doi.org/10.1097/01.ASW.0000526607.80113.66 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Journal Article |
| Item type description |
Article |
