Photobiomodulation Elicits a Differential Cytokine Response in a Cultured Analogue of Human Skin. (Record no. 4180)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02651nam a22003617a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 201229s20192019 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
| International Standard Serial Number | 1937-5719 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| PMID | 30858901 |
| 245 ## - TITLE STATEMENT | |
| Title | Photobiomodulation Elicits a Differential Cytokine Response in a Cultured Analogue of Human Skin. |
| 251 ## - Source | |
| Source | Eplasty [Electronic Resource]. 19:e3, 2019. |
| 252 ## - Abbreviated Source | |
| Abbreviated source | Eplasty. 19:e3, 2019. |
| 253 ## - Journal Name | |
| Journal name | Eplasty |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Year | 2019 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Manufacturer | FY2019 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] | |
| Publication status | epublish |
| 266 ## - Date added to catalog | |
| Date added to catalog | 2020-12-29 |
| 520 ## - SUMMARY, ETC. | |
| Abstract | Background: The study of photobiomodulation in wound healing is encumbered by limited wound study models. The aim of this study was to investigate the efficacy of a 3-dimensional dermal tissue culture model as a cost-saving alternative to conventional photobiomodulation study techniques. Methods: Nine dermal analogue tissue cultures were treated for 2 days with sham or 660-nm wavelength of light at either 1.5 or 3 mW/cm2 of energy. Tissue cytokine mRNA production was assessed by real-time reverse transcription-polymerase chain reaction, and tissue and supernatant protein were evaluated by immunofluorescence, enzyme-linked immunosorbent assay, and Western blot. Results: Photobiomodulation with 660-nm wavelength light induced transcription of IL-1beta and IL-6 mRNA and decreased that of IL-8. Tissue protein content of IL-6 and IL-8 was unchanged, whereas supernatant protein content of IL-8 was significantly increased (P = .023) by 1.5 mW/cm2 treatment. To describe the localization of cytokines between tissue and supernatant, the relative diffusion of each was calculated and found to be 15-fold higher for IL-6 than for IL-8 despite an overall higher concentration of IL-8 in the tissue. Conclusion: In this study, photobiomodulation elicited mRNA and protein changes quantifiable in both the tissue and supernatant. In addition, the use of this advanced culture model allowed for histological assessment and the comparison of "local" versus "circulatory" responses between the tissue and supernatant, respectively. |
| 546 ## - LANGUAGE NOTE | |
| Language note | English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME | |
| Institution | MedStar Health Research Institute |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME | |
| Institution | MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION | |
| Department | Firefighters' Burn and Surgical Research Laboratory |
| 656 ## - INDEX TERM--OCCUPATION | |
| Department | Surgery/Burn Services |
| 657 ## - INDEX TERM--FUNCTION | |
| Medline publication type | Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Ardanuy, Jeremy G |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Carney, Bonnie C |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Moffatt, Lauren T |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Prindeze, Nicholas J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Local Authors | Shupp, Jeffrey W |
| 790 ## - Authors | |
| All authors | Ardanuy JG, Carney BC, Moffatt LT, Prindeze NJ, Shupp JW |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journal Article |
| Item type description | Article |
| Withdrawn status | Lost status | Damaged status | Not for loan | Collection | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MedStar Authors Catalog | MedStar Authors Catalog | 12/29/2020 | 30858901 | 30858901 | 12/29/2020 | 12/29/2020 | Journal Article |