MARC details
| 000 -LEADER |
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| fixed length control field |
02595nam a22003257a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
190823s20192019 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1687-8450 |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
10.1155/2019/6486173 [doi] |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
PMC6662463 [pmc] |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
31379943 |
| 245 ## - TITLE STATEMENT |
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| Title |
CD133 Is Associated with Increased Melanoma Cell Survival after Multikinase Inhibition. |
| 251 ## - Source |
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| Source |
Journal of Oncology Print. 2019:6486173, 2019. |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
J. Oncol.. 2019:6486173, 2019. |
| 253 ## - Journal Name |
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| Journal name |
Journal of oncology |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2019 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2020 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
epublish |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2019-08-23 |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
FDA-approved kinase inhibitors are now used for melanoma, including combinations of the MEK inhibitor trametinib, and BRAF inhibitor dabrafenib for BRAFV600 mutations. NRAS-mutated cell lines are also sensitive to MEK inhibition in vitro, and NRAS-mutated tumors have also shown partial response to MEK inhibitors. However, melanoma still has high recurrence rates due to subpopulations, sometimes described as "melanoma initiating cells," resistant to treatment. Since CD133 is a putative cancer stem cell marker for different cancers, associated with decreased survival, we examined resistance of patient-derived CD133(+) and CD133(-) melanoma cells to MAPK inhibitors. Human melanoma cells were exposed to increasing concentrations of trametinib and/or dabrafenib, either before or after separation into CD133(+) and CD133(-) subpopulations. In parental CD133-mixed lines, the percentages of CD133(+) cells increased significantly (p<0.05) after high-dose drug treatment. Presorted CD133(+) cells also exhibited significantly greater (p<0.05) IC50s for single and combination MAPKI treatment. siRNA knockdown revealed a causal relationship between CD133 and drug resistance. Microarray and qRT-PCR analyses revealed that ten of 18 ABC transporter genes were significantly (P<0.05) upregulated in the CD133(+) subpopulation, while inhibition of ABC activity increased sensitivity, suggesting a mechanism for increased drug resistance of CD133(+) cells. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Franklin Square Medical Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Surgical Oncology |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
McCarron, Edward |
| 790 ## - Authors |
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| All authors |
AbdusSamad M, Aljehane L, Clark H, Dougherty R, Gaur A, Karna K, Kuo LW, McCarron E, Qin Q, Rosenthal DS, Simbulan-Rosenthal CM, Vakili S, Zhou H |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1155/2019/6486173">https://dx.doi.org/10.1155/2019/6486173</a> |
| Public note |
https://dx.doi.org/10.1155/2019/6486173 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Journal Article |
| Item type description |
Article |
