Microfluidic Droplet Digital PCR Is a Powerful Tool for Detection of BRAF and TERT Mutations in Papillary Thyroid Carcinomas. (Record no. 4877)
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fixed length control field | 02386nam a22003137a 4500 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | OSt |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20230312221700.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 200103s20192019 xxu||||| |||| 00| 0 eng d |
022 ## - INTERNATIONAL STANDARD SERIAL NUMBER | |
International Standard Serial Number | 2072-6694 |
024 ## - OTHER STANDARD IDENTIFIER | |
Standard number or code | 10.3390/cancers11121916 [doi] |
024 ## - OTHER STANDARD IDENTIFIER | |
Standard number or code | cancers11121916 [pii] |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | Ovid MEDLINE(R) |
Transcribing agency | whc |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
PMID | 31810221 |
245 ## - TITLE STATEMENT | |
Title | Microfluidic Droplet Digital PCR Is a Powerful Tool for Detection of BRAF and TERT Mutations in Papillary Thyroid Carcinomas. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Year | 2019 |
266 ## - Date added to catalog | |
Date added to catalog | 2020-01-03 |
520 ## - SUMMARY, ETC. | |
Abstract | We examined the utility of microfluidic digital PCR (dPCR) for detection of BRAF and TERT mutations in thyroid tumors. DNA extracted from 100 thyroid tumors (10 follicular adenomas, 10 follicular cancers, 5 medullary cancers, and 75 papillary thyroid cancer (PTC) were used for detection of BRAF and TERT mutations. Digital PCRs were performed using rare mutation SNP genotyping assays on QuantStudio 3D platform. In PTCs, BRAFV600E was detected by dPCR and Sanger sequencing in 42/75 (56%) and in 37/75 (49%), respectively. BRAFV600E was not detected in other tumors. The ratio of mutant/total BRAF alleles varied from 4.7% to 47.5%. These ratios were higher in classical PTCs (27.1%) as compared to follicular variant PTCs (9.4%) p = 0.001. In PTCs with and without metastases, the ratios of mutant/total BRAF alleles were 27.6% and 18.4%, respectively, (p = 0.03). In metastatic lesions percentages of mutant/total BRAF alleles were similar to those detected in primary tumors. TERTC228T and TERTC250T were found in two and one cases, respectively, and these tumors concomitantly harbored BRAFV600E. These tumors exhibited gross extra-thyroidal extension, metastases to lymph nodes, and pulmonary metastases (one case). Our results showed that dPCR allows quantitative assessment of druggable targets in PTCs and could be helpful in a molecular-based stratification of prognosis in patients with thyroid cancer. |
546 ## - LANGUAGE NOTE | |
Language note | English |
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | IN PROCESS -- NOT YET INDEXED |
9 (RLIN) | 7 |
651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME | |
Institution | MedStar Health Research Institute |
9 (RLIN) | 44 |
651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME | |
Institution | MedStar Washington Hospital Center |
9 (RLIN) | 10 |
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Department | Medicine/Endocrinology |
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Medline publication type | Journal Article |
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Local Authors | Burman, Kenneth D |
9 (RLIN) | 43 |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Local Authors | Gomes-Lima, Cristiane J |
9 (RLIN) | 41 |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Local Authors | Wartofsky, Leonard |
9 (RLIN) | 42 |
700 ## - ADDED ENTRY--PERSONAL NAME | |
Local Authors | Ylli, Dorina |
9 (RLIN) | 40 |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Journal Article |
Source of classification or shelving scheme | Dewey Decimal Classification |
Item type description | Article |
Withdrawn status | Lost status | Damaged status | Not for loan | Collection | Home library | Current library | Date acquired | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
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MedStar Authors Catalog | MedStar Authors Catalog | 01/03/2020 | 31810221 | 31810221 | 01/03/2020 | 01/03/2020 | Journal Article |