MARC details
| 000 -LEADER |
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| fixed length control field |
03138nam a22003977a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
201231s20202020 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0095-1137 |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
10.1128/JCM.02486-20 [doi] |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
JCM.02486-20 [pii] |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
33262219 |
| 245 ## - TITLE STATEMENT |
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| Title |
Pooled Saliva Specimens for SARS-CoV-2 Testing. |
| 251 ## - Source |
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| Source |
Journal of Clinical Microbiology. 2020 Dec 01 |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
J Clin Microbiol. 2020 Dec 01 |
| 253 ## - Journal Name |
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| Journal name |
Journal of clinical microbiology |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2020 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2021 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
aheadofprint |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2020-12-31 |
| 501 ## - WITH NOTE |
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| Local holdings |
Available online from MWHC library: 1975 - present (after 6 months), Available in print through MWHC library: 1999 - 2001 |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
We evaluated saliva (SAL) specimens for SARS-CoV-2 RT-PCR testing by comparison of 459 prospectively paired nasopharyngeal (NP) or mid-turbinate (MT) swabs from 449 individuals with the aim of using saliva for asymptomatic screening. Samples were collected in a drive-through car line for symptomatic individuals (N=380) and in the emergency department (ED) (N=69). The percent positive and negative agreement of saliva compared to nasopharyngeal swab were 81.1% (95% CI: 65.8% - 90.5%) and 99.8% (95% CI: 98.7% - 100%), respectively. The percent positive agreement increased to 90.0% (95% CI: 74.4% - 96.5%) when considering only samples with moderate to high viral load (Cycle threshold (Ct) for the NP <=34). Pools of five saliva specimens were also evaluated on three platforms: bioMerieux NucliSENS easyMAG with ABI 7500Fast (CDC assay), Hologic Panther Fusion R, and Roche COBAS R 6800. The average loss of signal upon pooling was 2-3 Ct values across the platforms. The sensitivity of detecting a positive specimen in a pool compared with testing individually was 94%, 90%, and 94% for CDC 2019-nCoV Real-Time RT-PCR, Panther Fusion R SARS-CoV-2 assay, and cobas R SARS-CoV-2 test respectively, with decreased sample detection trending with lower viral load. We conclude that although pooled saliva testing, as collected in this study, is not quite as sensitive as NP/MT testing, saliva testing is adequate to detect individuals with higher viral loads in an asymptomatic screening program, does not require swabs or viral transport media for collection, and may help to improve voluntary screening compliance for those individuals averse to various forms of nasal collections. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Health Research Institute |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Medicine/Infectious Diseases |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Nursing |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Moriarty, Theresa |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Sawney, Shari |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Tanjutco, Patricia |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Wortmann, Glenn W |
| 790 ## - Authors |
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| All authors |
Barat B, Das S, De Giorgi V, Frank KM, Henderson DK, Kopka S, Lau AF, Miller T, Moriarty T, Palmore TN, Sawney S, Spalding C, Tanjutco P, Wortmann G, Zelazny AM |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1128/JCM.02486-20">https://dx.doi.org/10.1128/JCM.02486-20</a> |
| Public note |
https://dx.doi.org/10.1128/JCM.02486-20 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Journal Article |
| Item type description |
Article |
