MARC details
| 000 -LEADER |
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| fixed length control field |
02849nam a22003377a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
210217s20212021 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1932-6203 |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
10.1371/journal.pone.0245243 [doi] |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
PONE-D-20-26287 [pii] |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
33444404 |
| 245 ## - TITLE STATEMENT |
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| Title |
Potential role of the skin and gut microbiota in premenarchal vulvar lichen sclerosus: A pilot case-control study. |
| 251 ## - Source |
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| Source |
PLoS ONE [Electronic Resource]. 16(1):e0245243, 2021. |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
PLoS ONE. 16(1):e0245243, 2021. |
| 253 ## - Journal Name |
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| Journal name |
PloS one |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2021 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Manufacturer |
FY2021 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
epublish |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2021-02-17 |
| 501 ## - WITH NOTE |
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| Local holdings |
Available online through MWHC library: 2006 - present |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
The etiology of vulvar lichen sclerosus (LS) remains unclear; however, alterations in cutaneous and gut microbiota may be contributing to the pathogenesis of this inflammatory condition. To explore this hypothesis, we conducted a pilot case-control study, obtaining dermal swab and stool samples from prepubertal girls with vulvar LS (n = 5), girls with nonspecific vulvovaginitis (n = 5), and healthy controls (n = 3). Samples (n = 56) were subjected to total DNA extractions. Resulting DNA was purified, subjected to PCR (targeting the V3V4 region of the 16S rRNA gene), sequenced, and analyzed using QIIME, MetagenomeSeq, and DESeq2 software packages. Our findings showed that there were significant differences in the cutaneous and gut microbiotas of girls with LS compared to controls. On the skin, girls with LS had a statistically significantly higher relative abundance of Porphyromonas spp., Parvimonas spp., Peptoniphilus spp., Prevotella spp., Dialister spp., and Peptostreptococcus spp., but a lower relative abundance of Cornyebacterium compared to the control group. In the gut samples, girls with LS had a significantly higher relative abundance of Dialister spp., Clostridiales spp., Paraprevotella spp., Escherichia coli, Bifidobacterium adolescentis, and Akkermansia muciniphila, and a lower relative abundance of Roseburia faecis and Ruminococcus bromii compared to controls. These results suggest a potential association between cutaneous and gut dysbiosis and pediatric vulvar LS. Future studies involving larger samples sizes are warranted to further evaluate this association. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Obstetrics and Gynecology/Pediatric and Adolescent |
| 657 ## - INDEX TERM--FUNCTION |
|---|
| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Gomez-Lobo, Veronica |
| 790 ## - Authors |
|---|
| All authors |
Arnold JD, Chattopadhyay S, Gomez-Lobo V, Hittle L, Malayil L, Marathe KS, Mongodin EF, Sapkota AR |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
|---|
| DOI |
<a href="https://dx.doi.org/10.1371/journal.pone.0245243">https://dx.doi.org/10.1371/journal.pone.0245243</a> |
| Public note |
https://dx.doi.org/10.1371/journal.pone.0245243 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Journal Article |
| Item type description |
Article |
