MARC details
| 000 -LEADER |
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| fixed length control field |
03270nam a22003737a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
211101s20212021 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
0017-5749 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
34340996 |
| 245 ## - TITLE STATEMENT |
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| Title |
The tumour microenvironment shapes innate lymphoid cells in patients with hepatocellular carcinoma. |
| 251 ## - Source |
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| Source |
Gut. 2021 Aug 02 |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
Gut. 2021 Aug 02 |
| 253 ## - Journal Name |
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| Journal name |
Gut |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2021 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2022 |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
aheadofprint |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2021-11-01 |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
CONCLUSION: Our results suggest that the tumour cytokine milieu controls ILC composition and HCC outcome. Specific changes of cytokines modify ILC composition in the tumour by inducing plasticity and alter ILC function. Copyright (c) Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
DESIGN: We performed bulk RNA sequencing of HCC tissue as well as flow cytometry and single-cell RNA sequencing of enriched ILCs from non-tumour liver, margin and tumour core derived from 48 patients with HCC. Simultaneous measurement of protein and RNA expression at the single-cell level (AbSeq) identified precise signatures of ILC subgroups. In vitro culturing of ILCs was used to validate findings from in silico analysis. Analysis of RNA-sequencing data from large HCC cohorts allowed stratification and survival analysis based on transcriptomic signatures. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
OBJECTIVE: Hepatocellular carcinoma (HCC) represents a typical inflammation-associated cancer. Tissue resident innate lymphoid cells (ILCs) have been suggested to control tumour surveillance. Here, we studied how the local cytokine milieu controls ILCs in HCC. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
RESULTS: RNA sequencing of tumour, non-tumour and margin identified tumour-dependent gradients, which were associated with poor survival and control of ILC plasticity. Single-cell RNA sequencing and flow cytometry of ILCs from HCC livers identified natural killer (NK)-like cells in the non-tumour tissue, losing their cytotoxic profile as they transitioned into tumour ILC1 and NK-like-ILC3 cells. Tumour ILC composition was mediated by cytokine gradients that directed ILC plasticity towards activated tumour ILC2s. This was liver-specific and not seen in ILCs from peripheral blood mononuclear cells. Patients with high ILC2/ILC1 ratio expressed interleukin-33 in the tumour that promoted ILC2 generation, which was associated with better survival. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
IN PROCESS -- NOT YET INDEXED |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
MedStar Georgetown University Hospital |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Surgery/Transplantation |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Transplant Surgery Research Fellowship |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Dhani, Harmeet |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Fishbein, Thomas M |
| 790 ## - Authors |
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| All authors |
Craig A, Dhani HS, Diggs LP, Fishbein T, Gertz EM, Greten TF, Heinrich B, Heinrich S, Hu Y, Kang J, Kapoor V, Kleiner DE, Korangy F, Kroemer A, Ma C, McVey JC, Rosato U, Ruf B, Ruppin E, Schaffer AA, Shen TW, Stovroff MK, Subramanyam V, Telford W, Wang XW, Yan C, Zhao Y |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1136/gutjnl-2021-325288">https://dx.doi.org/10.1136/gutjnl-2021-325288</a> |
| Public note |
https://dx.doi.org/10.1136/gutjnl-2021-325288 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Journal Article |
| Item type description |
Article |
