Intraoperative Fluorescein Staining of Cryopreserved Amniotic Membrane Grafts to Improve Visualization During and After Pterygium Surgery: A Novel Technique.

MedStar author(s):
Citation: Cornea. 35(3):413-6, 2016 Mar.PMID: 26751995Institution: MedStar Washington Hospital CenterDepartment: OphthalmologyForm of publication: Journal ArticleMedline article type(s): Journal Article | Research Support, Non-U.S. Gov'tSubject headings: *Amnion/tr [Transplantation] | *Fluorescein/ad [Administration & Dosage] | *Fluorescent Dyes/ad [Administration & Dosage] | *Ophthalmologic Surgical Procedures/mt [Methods] | *Pterygium/su [Surgery] | Adult | Female | Humans | Intraoperative Period | Male | Retrospective StudiesYear: 2016Local holdings: Available online from MWHC library: 2000 - presentISSN:
  • 0277-3740
Name of journal: CorneaAbstract: CONCLUSIONS: Fluorescein strip staining of the amniotic membrane is a novel and safe intraoperative method to enhance visualization and handling of the graft during and after ocular surgeries.METHODS: Pterygium excision surgery using intraoperatively stained cryopreserved amniotic membranes was performed on 346 eyes. A sterile 0.6 mg sodium fluorescein strip was placed directly onto the amniotic membrane in the manufacturer's original packaging, and the stained allograft was then transplanted onto the planned site. Staining intensities, at 3, 5, and 10 minutes of dye immersion, were compared. Immediate postoperative pain rating (scale 0-10), visibility of the fluorescein-stained amniotic membrane graft, and conjunctival autograft and amniotic membrane graft elevation, dehiscence, retraction, or displacement were recorded. The recurrence rate of the study population was compared with that of a previous cohort of 121 patients who underwent pterygium excision with conjunctival autograft without stained amniotic membrane.PURPOSE: To describe a new method of enhancing the visualization of amniotic membrane grafts with fluorescein staining during pterygium surgery.RESULTS: Direct contact of the fluorescein strip on the amniotic membrane at 3, 5, and 10 minutes showed no differences in subjective staining intensity. Fluorescein-stained amniotic membrane was easily detected on the ocular surface during and 24 hours after pterygium surgery. The average immediate postoperative pain rating was 0.8 +/- 1.8. No intraoperative complications or postoperative amniotic membrane graft dehiscence, retraction, or displacement occurred. The recurrence rate using fluorescein-stained amniotic membrane (3 patients, 0.9%, mean follow-up time 31.8 +/- 18.6 weeks) did not differ from that of the previous cohort without the stained amniotic membrane (2.5%; chi(1) = 1.837, P = 0.183).All authors: Cremers SL, Ha J, Korchak M, Martinez JAFiscal year: FY2016Digital Object Identifier: Date added to catalog: 2017-03-07
Holdings
Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 26751995 Available 26751995

Available online from MWHC library: 2000 - present

CONCLUSIONS: Fluorescein strip staining of the amniotic membrane is a novel and safe intraoperative method to enhance visualization and handling of the graft during and after ocular surgeries.

METHODS: Pterygium excision surgery using intraoperatively stained cryopreserved amniotic membranes was performed on 346 eyes. A sterile 0.6 mg sodium fluorescein strip was placed directly onto the amniotic membrane in the manufacturer's original packaging, and the stained allograft was then transplanted onto the planned site. Staining intensities, at 3, 5, and 10 minutes of dye immersion, were compared. Immediate postoperative pain rating (scale 0-10), visibility of the fluorescein-stained amniotic membrane graft, and conjunctival autograft and amniotic membrane graft elevation, dehiscence, retraction, or displacement were recorded. The recurrence rate of the study population was compared with that of a previous cohort of 121 patients who underwent pterygium excision with conjunctival autograft without stained amniotic membrane.

PURPOSE: To describe a new method of enhancing the visualization of amniotic membrane grafts with fluorescein staining during pterygium surgery.

RESULTS: Direct contact of the fluorescein strip on the amniotic membrane at 3, 5, and 10 minutes showed no differences in subjective staining intensity. Fluorescein-stained amniotic membrane was easily detected on the ocular surface during and 24 hours after pterygium surgery. The average immediate postoperative pain rating was 0.8 +/- 1.8. No intraoperative complications or postoperative amniotic membrane graft dehiscence, retraction, or displacement occurred. The recurrence rate using fluorescein-stained amniotic membrane (3 patients, 0.9%, mean follow-up time 31.8 +/- 18.6 weeks) did not differ from that of the previous cohort without the stained amniotic membrane (2.5%; chi(1) = 1.837, P = 0.183).

English

Powered by Koha