CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis.

MedStar author(s):
Citation: Journal of Clinical Investigation. 128(3):1106-1124, 2018 03 01.PMID: 29457790Institution: MedStar Health Research Institute | MedStar Heart & Vascular InstituteForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Antigens, CD/me [Metabolism] | *Antigens, Differentiation, Myelomonocytic/me [Metabolism] | *Atherosclerosis/me [Metabolism] | *Inflammation/me [Metabolism] | *Macrophages/cy [Cytology] | *Neovascularization, Pathologic | *Receptors, Cell Surface/me [Metabolism] | Adult | Animals | Antigens, CD/ge [Genetics] | Antigens, Differentiation, Myelomonocytic/ge [Genetics] | Coronary Disease/me [Metabolism] | Coronary Vessels/me [Metabolism] | Disease Progression | Female | Hemoglobins/me [Metabolism] | Humans | Macrophages/me [Metabolism] | Male | Mice | Mice, Inbred C57BL | Middle Aged | Myocardial Infarction/me [Metabolism] | Oxidative Stress | Permeability | Phenotype | Polymorphism, Single Nucleotide | Receptors, Cell Surface/ge [Genetics] | Signal TransductionYear: 2018Local holdings: Available online from MWHC library: 1924 - present, Available in print through MWHC library: 1999 - March 2001ISSN:
  • 0021-9738
Name of journal: The Journal of clinical investigationAbstract: Intake of hemoglobin by the hemoglobin-haptoglobin receptor CD163 leads to a distinct alternative non-foam cell antiinflammatory macrophage phenotype that was previously considered atheroprotective. Here, we reveal an unexpected but important pathogenic role for these macrophages in atherosclerosis. Using human atherosclerotic samples, cultured cells, and a mouse model of advanced atherosclerosis, we investigated the role of intraplaque hemorrhage on macrophage function with respect to angiogenesis, vascular permeability, inflammation, and plaque progression. In human atherosclerotic lesions, CD163+ macrophages were associated with plaque progression, microvascularity, and a high level of HIF1alpha and VEGF-A expression. We observed irregular vascular endothelial cadherin in intraplaque microvessels surrounded by CD163+ macrophages. Within these cells, activation of HIF1alpha via inhibition of prolyl hydroxylases promoted VEGF-mediated increases in intraplaque angiogenesis, vascular permeability, and inflammatory cell recruitment. CD163+ macrophages increased intraplaque endothelial VCAM expression and plaque inflammation. Subjects with homozygous minor alleles of the SNP rs7136716 had elevated microvessel density, increased expression of CD163 in ruptured coronary plaques, and a higher risk of myocardial infarction and coronary heart disease in population cohorts. Thus, our findings highlight a nonlipid-driven mechanism by which alternative macrophages promote plaque angiogenesis, leakiness, inflammation, and progression via the CD163/HIF1alpha/VEGF-A pathway.All authors: Akahori H, Arking DE, Boerwinkle E, Braumann RE, Cao Y, Cheng Q, Chhour P, Choi CU, Cormode DP, de Vries PS, Dickinson MH, Erdmann J, Finn AV, Gannon RL, Grove ML, Guo L, Gupta A, Harari E, Jenkins AL, Jinnouchi H, Karmali V, Kim J, Kolodgie FD, Kutyna MD, Kutys R, Lipinski MJ, Mori H, Morrison AC, Otsuka F, Polavarapu R, Sakamoto A, Sawan MA, Smith SL, Sotoodehnia N, Torii S, Virmani R, Zhang YFiscal year: FY2018Digital Object Identifier: Date added to catalog: 2018-02-28
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Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 29457790 Available 29457790

Available online from MWHC library: 1924 - present, Available in print through MWHC library: 1999 - March 2001

Intake of hemoglobin by the hemoglobin-haptoglobin receptor CD163 leads to a distinct alternative non-foam cell antiinflammatory macrophage phenotype that was previously considered atheroprotective. Here, we reveal an unexpected but important pathogenic role for these macrophages in atherosclerosis. Using human atherosclerotic samples, cultured cells, and a mouse model of advanced atherosclerosis, we investigated the role of intraplaque hemorrhage on macrophage function with respect to angiogenesis, vascular permeability, inflammation, and plaque progression. In human atherosclerotic lesions, CD163+ macrophages were associated with plaque progression, microvascularity, and a high level of HIF1alpha and VEGF-A expression. We observed irregular vascular endothelial cadherin in intraplaque microvessels surrounded by CD163+ macrophages. Within these cells, activation of HIF1alpha via inhibition of prolyl hydroxylases promoted VEGF-mediated increases in intraplaque angiogenesis, vascular permeability, and inflammatory cell recruitment. CD163+ macrophages increased intraplaque endothelial VCAM expression and plaque inflammation. Subjects with homozygous minor alleles of the SNP rs7136716 had elevated microvessel density, increased expression of CD163 in ruptured coronary plaques, and a higher risk of myocardial infarction and coronary heart disease in population cohorts. Thus, our findings highlight a nonlipid-driven mechanism by which alternative macrophages promote plaque angiogenesis, leakiness, inflammation, and progression via the CD163/HIF1alpha/VEGF-A pathway.

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