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Detection of RAS and RAS-associated alterations in primary lung adenocarcinomas. A correlation between molecular findings and tumor characteristics.

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Citation: Human Pathology. 2018 Sep 25PMID: 30266251Institution: MedStar Good Samaritan HospitalDepartment: MedicineForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: IN PROCESS -- NOT YET INDEXEDYear: 2018 Local holdings: Available online from MWHC library: 1995 - present, Available in print through MWHC library:1999-2007ISSN:

0046-8177

Name of journal: Human pathologyAbstract: Copyright (c) 2018. Published by Elsevier Inc.Rat sarcoma (RAS) and RAS-associated pathways play important roles in the pathogenesis of lung cancers as well as in the development of targeted-therapies. However, the clinical significance of RAS pathways is still not fully understood. We investigated the RAS-associated molecular aberrations in primary lung adenocarcinomas (ADCs), and correlated molecular findings with clinico-pathological characteristics of tumors. A total of 220 surgically resected tumors were identified for which a lung cancer molecular panel (testing seven genes by next generation sequencing (NGS) and three genes for rearrangement by fluorescence in situ hybridization (FISH)) had been performed. The overall molecular alterations were detected in 143 cases (65.00%), including 58 cases (26.36%) of Kirsten Rat Sarcoma viral oncogene (KRAS), 40 cases (18.18%) of Epidermal Growth Factor Receptor (EGFR), 24 cases (10.91%) of V-raf murine sarcoma viral oncogene homolog B (BRAF), 8 cases (3.64%) of phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), 7 cases (3.18%) of Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), 6 cases (2.73%) of Anaplastic Lymphoma Kinase (ALK) alterations. KRAS, BRAF NRAS and PIK3CA mutations were more commonly seen in smokers, and occurred with much higher rates than previously published data. BRAFV600E mutations were commonly seen in female smokers, whereas, BRAFnon-V600E mutations were seen in both male and female smokers with moderately- to poorly-differentiated tumors. PIK3CA mutations were predominantly occurred in p.E545K and p.E542K on exon 9 in moderately- to poorly differentiated tumors.All authors: Askin F, Gabrielson E, Hoti N, Kern JJ, Li J, Li QK, Shaukat I, Zhang H, Zheng GFiscal year: FY2019Digital Object Identifier:

https://dx.doi.org/10.1016/j.humpath.2018.09.004

Date added to catalog: 2018-10-10

Holdings ( 1 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Journal Article	MedStar Authors Catalog	Article	30266251	Available		30266251

Available online from MWHC library: 1995 - present, Available in print through MWHC library:1999-2007

Rat sarcoma (RAS) and RAS-associated pathways play important roles in the pathogenesis of lung cancers as well as in the development of targeted-therapies. However, the clinical significance of RAS pathways is still not fully understood. We investigated the RAS-associated molecular aberrations in primary lung adenocarcinomas (ADCs), and correlated molecular findings with clinico-pathological characteristics of tumors. A total of 220 surgically resected tumors were identified for which a lung cancer molecular panel (testing seven genes by next generation sequencing (NGS) and three genes for rearrangement by fluorescence in situ hybridization (FISH)) had been performed. The overall molecular alterations were detected in 143 cases (65.00%), including 58 cases (26.36%) of Kirsten Rat Sarcoma viral oncogene (KRAS), 40 cases (18.18%) of Epidermal Growth Factor Receptor (EGFR), 24 cases (10.91%) of V-raf murine sarcoma viral oncogene homolog B (BRAF), 8 cases (3.64%) of phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), 7 cases (3.18%) of Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS), 6 cases (2.73%) of Anaplastic Lymphoma Kinase (ALK) alterations. KRAS, BRAF NRAS and PIK3CA mutations were more commonly seen in smokers, and occurred with much higher rates than previously published data. BRAFV600E mutations were commonly seen in female smokers, whereas, BRAFnon-V600E mutations were seen in both male and female smokers with moderately- to poorly-differentiated tumors. PIK3CA mutations were predominantly occurred in p.E545K and p.E542K on exon 9 in moderately- to poorly differentiated tumors.

English