Citation: Experimental & Molecular Pathology. 104(1):82-88, 2018 Feb.Journal: Experimental and molecular pathology.Published: 2018ISSN: 0014-4800.Full author list: Mitchell R; Molligan J; Rooney S; Cho Y; Schon L; Zhang Z.UI/PMID: 29353045.Subject(s): Adipogenesis/ph [Physiology] | *Arthropathy, Neurogenic/pa [Pathology] | Cell Differentiation/ph [Physiology] | Cells, Cultured | Chondrogenesis/ph [Physiology] | Female | Foot/pa [Pathology] | Humans | Male | *Mesenchymal Stromal Cells/cy [Cytology] | Mesenchymal Stromal Cells/pa [Pathology] | Middle Aged | Osteogenesis/ph [Physiology] | *Synovial Membrane/cy [Cytology] | Synovial Membrane/pa [Pathology]Institution(s): MedStar Union Memorial HospitalDepartment(s): Orthopaedic Surgery | Orthobiologic LaboratoryActivity type: Journal Article.Medline article type(s): Journal ArticleDigital Object Identifier: https://dx.doi.org/10.1016/j.yexmp.2018.01.003 (Click here)Abbreviated citation: Exp Mol Pathol. 104(1):82-88, 2018 Feb.Abstract: Charcot neuroarthropathy (CNA) often presents as a diabetic foot complication. The role of synovial mesenchymal stem cells (syn-MSCs) in the pathogenesis of CNA is unclear. Synovial samples were collected, for isolation of syn-MSCs, from diabetic patients with CNA (n=7) and non-diabetic patients with intra-articular fracture or normal joints (non-CNA; n=7) during foot surgery. The syn-MSCs in the CNA and non-CNA groups were characterized comparatively. The average number of colonies formed in the CNA group was 6+/-3.5 per half plate (10mm in diameter), while it was 43+/-21.6 in the non-CNA group (p<0.05). The average size (pixels) of the colonies in the CNA group was smaller than that in the non-CNA group. When the colonies were stratified into high-, medium- and low-density subgroups, colonies in the high-density subgroup of the CNA group were reduced in density. Expression of PPAR-gamma, RUNX2, Sox9 and type II collagen by syn-MSCs in the CNA group was decreased during adipogenic, osteogenic and chondrogenic differentiation as compared with the non-CNA group. In conclusion, syn-MSCs in CNA joints were reduced in number, with declined differentiation potentials. The high-density subpopulation of the syn-MSCs was particularly affected by the pathology of CNA.Abstract: Copyright (c) 2018 Elsevier Inc. All rights reserved.