Plasma and Urinary Glycosaminoglycans as Evidence for Endotheliopathy in a Swine Burn Model.

MedStar author(s):
Citation: Journal of Surgical Research. 248:28-37, 2020 04.PMID: 31841734Institution: MedStar Health Research InstituteDepartment: Firefighters' Burn and Surgical Research LaboratoryForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Burns/me [Metabolism] | *Glycocalyx/ph [Physiology] | *Glycosaminoglycans/an [Analysis] | Acute Kidney Injury/di [Diagnosis] | Acute Kidney Injury/th [Therapy] | Animals | Disease Models, Animal | Endothelial Cells/ph [Physiology] | Glycosaminoglycans/bl [Blood] | Glycosaminoglycans/ur [Urine] | Kidney Tubules/pa [Pathology] | Lectins/an [Analysis] | Rehydration Solutions/tu [Therapeutic Use] | Swine | Syndecan-1/an [Analysis]Year: 2020ISSN:
  • 0022-4804
Name of journal: The Journal of surgical researchAbstract: BACKGROUND: The endothelial glycocalyx controls vascular permeability, cellular signaling, blood-endothelial cell adhesion, extravasation, and transmission of shear stress signals. Burn injury compromises integrity of this layer increasing vascular permeability, which is further exacerbated by large volumes of (intravenous) crystalloids. We have shown that enteral resuscitation is able to reverse burn-induced acute kidney injury (AKI), and herein, we present a follow-up examination of the integrity of the glycocalyx layer and its relationship with renal dysfunction after burn injury.CONCLUSIONS: Collectively, these data demonstrate that ORS prevented increases in circulating GAGs. Furthermore, an inexpensive and simple method for detecting GAGs provides a sensitive measure of endotheliopathy after burn. Copyright Published by Elsevier Inc.MATERIALS AND METHODS: Anesthetized Yorkshire pigs sustained 40% total body surface area full-thickness contact burns and recovered in metabolic cages for one of three treatments: no fluids (oral or intravenous); (n = 6), ad libitum water (n = 6), or volume-matched oral rehydration solution (ORS; n = 6) for 48 h. Urine and blood were collected at baseline (BL), 6, 12, 24, 32, and 48 h after burn at which point kidneys were harvested.RESULTS: In no fluid and water groups (but not ORS), plasma levels of glycosaminoglycans (GAGs) were elevated after burn (P <= 0.031). Syndecan-1 was elevated by 6 h after burn in all animals, but levels declined by 24 h with enteral fluids. Urinary GAGs in the no-fluid group were elevated after burn. No differences among treatments were detected in syndecan-1 levels, or glomerular lectin within the kidney.All authors: Burmeister DM, Dubick MA, Gomez BI, Schmidt EP, Shupp JWOriginally published: Journal of Surgical Research. 248:28-37, 2019 Dec 13.Fiscal year: FY2020Digital Object Identifier: Date added to catalog: 2020-01-03
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BACKGROUND: The endothelial glycocalyx controls vascular permeability, cellular signaling, blood-endothelial cell adhesion, extravasation, and transmission of shear stress signals. Burn injury compromises integrity of this layer increasing vascular permeability, which is further exacerbated by large volumes of (intravenous) crystalloids. We have shown that enteral resuscitation is able to reverse burn-induced acute kidney injury (AKI), and herein, we present a follow-up examination of the integrity of the glycocalyx layer and its relationship with renal dysfunction after burn injury.

CONCLUSIONS: Collectively, these data demonstrate that ORS prevented increases in circulating GAGs. Furthermore, an inexpensive and simple method for detecting GAGs provides a sensitive measure of endotheliopathy after burn. Copyright Published by Elsevier Inc.

MATERIALS AND METHODS: Anesthetized Yorkshire pigs sustained 40% total body surface area full-thickness contact burns and recovered in metabolic cages for one of three treatments: no fluids (oral or intravenous); (n = 6), ad libitum water (n = 6), or volume-matched oral rehydration solution (ORS; n = 6) for 48 h. Urine and blood were collected at baseline (BL), 6, 12, 24, 32, and 48 h after burn at which point kidneys were harvested.

RESULTS: In no fluid and water groups (but not ORS), plasma levels of glycosaminoglycans (GAGs) were elevated after burn (P <= 0.031). Syndecan-1 was elevated by 6 h after burn in all animals, but levels declined by 24 h with enteral fluids. Urinary GAGs in the no-fluid group were elevated after burn. No differences among treatments were detected in syndecan-1 levels, or glomerular lectin within the kidney.

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