Cutaneous Thermal Injury Modulates Blood and Skin Metabolomes Differently in a Murine Model.

MedStar author(s):
Citation: Journal of Burn Care & Research. 42(4):727-742, 2021 08 04.PMID: 33301570Institution: MedStar Health Research Institute | MedStar Washington Hospital CenterDepartment: Firefighters' Burn and Surgical Research Laboratory | Surgery/Burn ServicesForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Biomarkers/me [Metabolism] | *Burns/me [Metabolism] | *Hot Temperature | *Metabolome | Animals | Burns/pa [Pathology] | Disease Models, Animal | Metabolomics/mt [Methods] | MiceYear: 2021Local holdings: Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - presentISSN:
  • 1559-047X
Name of journal: Journal of burn care & research : official publication of the American Burn AssociationAbstract: As the field of metabolomics develops further, investigations of how the metabolome is affected following thermal injury may be helpful to inform diagnostics and guide treatments. In this study changes to the metabolome were tested and validated in a murine burn injury model. After a 30% total body surface scald injury or sham procedure sera and skin biopsies were collected at 1, 2, 6, or 24 hours. Burn-specific changes in the metabolome were detected compared to sham animals. The sera metabolome exhibited a more rapid response to burn injury than that of the skin and it peaked more proximal to injury (6 vs 24 hours). Progression of metabolic response in the skin was less synchronous and showed a higher overlap of the significantly modified metabolites (SMMs) among tested time-points. Top affected pathways identified by SMMs of skin included inositol phosphate metabolism, ascorbate and alderate metabolism, caffeine metabolism, and the pentose phosphate pathway. Future research is warranted in human and larger animal models to further elucidate the role of metabolomic perturbations and the pathophysiology following burn injury. Copyright (c) The Author(s) 2020. Published by Oxford University Press on behalf of the American Burn Association.All authors: Alkhalil A, Ball RL, Carney BC, Day A, Garg G, Hammamieh R, Kumar R, Moffatt LT, Shupp JWOriginally published: Journal of Burn Care & Research. 2020 Dec 10Fiscal year: FY2022Fiscal year of original publication: FY2021Digital Object Identifier: Date added to catalog: 2020-12-31
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Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 33301570 Available 33301570

Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present

As the field of metabolomics develops further, investigations of how the metabolome is affected following thermal injury may be helpful to inform diagnostics and guide treatments. In this study changes to the metabolome were tested and validated in a murine burn injury model. After a 30% total body surface scald injury or sham procedure sera and skin biopsies were collected at 1, 2, 6, or 24 hours. Burn-specific changes in the metabolome were detected compared to sham animals. The sera metabolome exhibited a more rapid response to burn injury than that of the skin and it peaked more proximal to injury (6 vs 24 hours). Progression of metabolic response in the skin was less synchronous and showed a higher overlap of the significantly modified metabolites (SMMs) among tested time-points. Top affected pathways identified by SMMs of skin included inositol phosphate metabolism, ascorbate and alderate metabolism, caffeine metabolism, and the pentose phosphate pathway. Future research is warranted in human and larger animal models to further elucidate the role of metabolomic perturbations and the pathophysiology following burn injury. Copyright (c) The Author(s) 2020. Published by Oxford University Press on behalf of the American Burn Association.

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