MedStar Authors catalog › Details for: Shedding of the endothelial glycocalyx is quantitatively proportional to burn injury severity.
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Shedding of the endothelial glycocalyx is quantitatively proportional to burn injury severity.

by Luker, Jenna N; Vigiola Cruz, Mariana; Carney, Bonnie C; Day, Anna; Moffatt, Lauren T; Johnson, Laura S; Shupp, Jeffrey W.
Citation: Annals of Burns & Fire Disasters. 31(1):17-22, 2018 Mar 31..Journal: Annals of burns and fire disasters.Published: ; 2018ISSN: 1592-9558.Full author list: Luker JN; Vigiola Cruz M; Carney BC; Day A; Moffatt LT; Johnson LS; Shupp JW.UI/PMID: 30174566.Subject(s): IN PROCESS -- NOT YET INDEXEDInstitution(s): MedStar Washington Hospital Center | MedStar Health Research InstituteDepartment(s): Surgery/Burn Services | Firefighters' Burn and Surgical Research LaboratoryActivity type: Journal Article.Medline article type(s): Journal ArticleOnline resources: Click here to access online Abbreviated citation: Ann. Burns Fire Disasters. 31(1):17-22, 2018 Mar 31.Abstract: Limited information exists regarding endothelial dysfunction following burn injury. This project aims to evaluate whether thermal injury results in shedding of the endothelial glycocalyx in a manner quantitatively proportional to injury severity, and whether theloss of intact glycocalyx is measurable in end organs. C57BL/6 mice were grouped as uninjured controls, 10% or 25% Total Body Surface Area (TBSA) scald burns. Blood and tissue sampling was performed over a specific time course. Plasma levels of shed syndecan-1, a marker of glycocalyx damage, were quantified by ELISA. Lung and spleen sections were stained with immunofluorescent anti-syndecan-1 antibodies to evaluate intact glycocalyx. Plasma syndecan-1 levels were higher in injured versus uninjured animals. Normalized levels of syndecan-1 in burned mice were significantly increased compared to hour 0 (p<0.05) at hours 4 and 8 post-injury in the 10% TBSA, and at hour 4 in the 25% TBSA group. Levels in the 10% and 25% TBSA groups peaked at hour 4 with fold change of 2.3 and 2.4 respectively. There was less pulmonary syndecan-1 immunostaining in burned animals compared to controls, and the levels inversely correlated with systemic shed syndecan- 1, beginning at hour 4 in the 10% TBSA injury group and at all time points in the 25% TBSA injury group, (0.27+/-0.06 and 0.14+/-0.04 respectively for hour 4). Similarly, there was less spleen syndecan-1 immunostaining in burned animals compared to controls at all time points. Burn injury causes shedding of syndecan-1 in a murine model, with levels correlated to injury severity and loss of the glycocalyx in lung and spleen. This work provides further insight into quantification and temporality of glycocalyx damage and systemic response to burn.

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