MedStar Authors catalog › Details for: Plasma Ameliorates Endothelial Dysfunction in Burn Injury.
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Plasma Ameliorates Endothelial Dysfunction in Burn Injury.

by Vigiola Cruz, Mariana; Carney, Bonnie C; Luker, Jenna N; Monger, Kyle W; Vazquez, Juan Sebastian; Moffatt, Lauren T; Johnson, Laura; Shupp, Jeffrey W.
Citation: Journal of Surgical Research. 233:459-466, 2019 Jan..Journal: The Journal of surgical research.Published: ; 2019ISSN: 0022-4804.Full author list: Vigiola Cruz M; Carney BC; Luker JN; Monger KW; Vazquez JS; Moffatt LT; Johnson LS; Shupp JW.UI/PMID: 30502286.Subject(s): IN PROCESS -- NOT YET INDEXEDInstitution(s): MedStar Washington Hospital Center | MedStar Health Research InstituteDepartment(s): Surgery/Burn Services | Firefighters' Burn and Surgical Research LaboratoryActivity type: Journal Article.Medline article type(s): Journal ArticleOnline resources: Click here to access online Digital Object Identifier: (Click here) Abbreviated citation: J Surg Res. 233:459-466, 2019 Jan.Abstract: BACKGROUND: A complex inflammatory response mediates the systemic effects of burn shock. Disruption of the endothelial glycocalyx causes shedding of structural glycoproteins, primarily syndecan-1 (SDC-1), leading to endothelial dysfunction. These effects may be mitigated by resuscitative interventions.Abstract: MATERIALS AND METHODS: Sprague-Dawley rats were used to create small, medium, and large burns and uninjured controls. Three different intravenous resuscitation protocols were applied within each group: Lactated Ringer's (LR) alone, LR plus fresh frozen plasma (FFP), or LR plus albumin. Blood was serially collected, and plasma SDC-1 was quantified with enzyme-linked immunosorbent assay. In one cohort, Evan's Blue Dye (EBD) was administered and quantified in lung by spectrophotometry as a functional assay of vascular permeability. In a second cohort, intact SCD-1 was quantified by immunohistochemistry in lung tissue. Statistical analysis employed two-way analysis of variance with multiple comparisons and Student's t-test.Abstract: RESULTS: EBD extraction from lung was significantly greater with higher injury severity versus controls. Extraction decreased significantly in large-burn animals with addition of FFP to LR versus LR-only; addition of albumin to LR did not decrease EBD extraction. Plasma SCD-1 increased in injured animals compared with controls, and changes correlated with injury severity in all resuscitation groups (significance, P < 0.05). Lung SCD-1 staining reflected the results in the EBD assay.Abstract: CONCLUSIONS: Addition of FFP, not of albumin, to post-burn resuscitation diminishes vascular leakage associated with large burns. Addition of colloid does not affect SDC-1 shedding as measured in plasma. Ongoing work will further define pathophysiologic mechanisms and potential therapeutic interventions to mitigate injury and promote repair of the endothelial glycocalyx.Abstract: Copyright (c) 2018 Elsevier Inc. All rights reserved.

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