TY  - BOOK
AU  - Alkhalil, Abdulnaser
AU  - Carney, Bonnie C
AU  - Kirkpatrick, Liam D
AU  - Moffatt, Lauren T
AU  - Shupp, Jeffrey W
AU  - Smith, Robert D
TI  - Galectin-1 production is elevated in hypertrophic scar
SN  - 1067-1927
PY  - 2021///
KW  - *Cicatrix, Hypertrophic/ge [Genetics]
KW  - *Galectin 1/bi [Biosynthesis]
KW  - *RNA, Messenger/ge [Genetics]
KW  - *Wound Healing
KW  - Animals
KW  - Biomarkers/me [Metabolism]
KW  - Cell Differentiation
KW  - Cicatrix, Hypertrophic/me [Metabolism]
KW  - Cicatrix, Hypertrophic/pa [Pathology]
KW  - Disease Models, Animal
KW  - Fibroblasts/me [Metabolism]
KW  - Fibroblasts/pa [Pathology]
KW  - Humans
KW  - Male
KW  - Swine
KW  - MedStar Health Research Institute
KW  - MedStar Washington Hospital Center
KW  - Firefighters' Burn and Surgical Research Laboratory
KW  - Surgery/Burn Services
KW  - Journal Article
N2  - Upon healing, burn wounds often leave hypertrophic scars (HTSs) marked by excess collagen deposition, dermal and epidermal thickening, hypervascularity, and an increased density of fibroblasts. The Galectins, a family of lectins with a conserved carbohydrate recognition domain, function intracellularly and extracellularly to mediate a multitude of biological processes including inflammatory responses, angiogenesis, cell migration and differentiation, and cell-ECM adhesion. Galectin-1 (Gal-1) has been associated with several fibrotic diseases and can induce keratinocyte and fibroblast proliferation, migration, and differentiation into fibroproliferative myofibroblasts. In this study, Gal-1 expression was assessed in human and porcine HTS. In a microarray, galectins 1, 4, and 12 were upregulated in pig HTS compared to normal skin (fold change = +3.58, +6.11, and +3.03, FDR <0.01). Confirmatory qRT-PCR demonstrated significant upregulation of Galectin-1 (LGALS1) transcription in HTS in both human and porcine tissues (fold change = +7.78 and +7.90, P <.05). In pig HTS, this upregulation was maintained throughout scar development and remodeling. Immunofluorescent staining of Gal-1 in human and porcine HTS showed significantly increased fluorescence (202.5 +/- 58.2 vs 35.2 +/- 21.0, P <.05 and 276.1 +/- 12.7 vs 69.7 +/- 25.9, P <.01) compared to normal skin and co-localization with smooth muscle actin-expressing myofibroblasts. A strong positive correlation (R = .948) was observed between LGALS1 and Collagen type 1 alpha 1 mRNA expression. Gal-1 is overexpressed in HTS at the mRNA and protein levels and may have a role in the development of scar phenotypes due to fibroblast over-proliferation, collagen secretion, and dermal thickening. The role of galectins shows promise for future study and may lead to the development of a pharmacotherapy for treatment of HTS. Copyright (c) 2020 The Wound Healing Society
UR  - https://dx.doi.org/10.1111/wrr.12869
ER  -