TY  - BOOK
AU  - Reddy, Uma M
TI  - Maternal serum serpin B7 is associated with early spontaneous preterm birth
SN  - 0002-9378
PY  - 2014///
KW  - *Gestational Age
KW  - *Obstetric Labor, Premature/bl [Blood]
KW  - *Premature Birth/bl [Blood]
KW  - *Serpins/bl [Blood]
KW  - Adult
KW  - Biological Markers/bl [Blood]
KW  - Case-Control Studies
KW  - Cohort Studies
KW  - Female
KW  - Humans
KW  - Longitudinal Studies
KW  - Pregnancy
KW  - Pregnancy Trimester, Second/bl [Blood]
KW  - Pregnancy Trimester, Third/bl [Blood]
KW  - Prospective Studies
KW  - Proteome
KW  - Proteomics
KW  - Young Adult
KW  - MedStar Washington Hospital Center
KW  - Obstetrics and Gynecology, Maternal-Fetal Medicine
KW  - Journal Article
KW  - Observational Study
KW  - Research Support, N.I.H., Extramural
N1  - Available online from MWHC library: 1995 - present, Available in print through MWHC library: 1999 - 2006
N2  - CONCLUSION: Targeted and shotgun serum proteomics analyses associated 1 protein, serpin B7, with early SPTB. Our results require validation in other cohorts and analysis of the possible mechanistic role of serpin B7 in parturition.Copyright � 2014 Elsevier Inc. All rights reserved; OBJECTIVE: We sought to identify serum biomarkers of early spontaneous preterm birth (SPTB) using semiquantitative proteomic analyses; RESULTS: Serum samples were available for 35 cases and 35 controls at 19-24 weeks, and 16 cases and 16 controls at 28-32 weeks. One protein, serpin B7, yielded serum concentrations that differed between cases and controls. The mean concentration of serpin B7 at 28-32 weeks was 1.5-fold higher in women with subsequent preterm deliveries compared to controls; there was no difference at 19-24 weeks. Higher levels of serpin B7 at both gestational age windows were associated with a shorter interval to delivery, and higher levels of serpin B7 in samples from 28-32 weeks were associated with a lower gestational age at delivery. Western blotting identified serpin B7 protein in placenta, amnion, and chorion from cases and controls; STUDY DESIGN: This was a nested case-control study of pregnant women with previous SPTB. Maternal serum was collected at 19-24 and 28-32 weeks' gestation, and analyzed by liquid chromatography-multiple reaction monitoring/mass spectrometry. Targeted and shotgun proteomics identified 31 candidate proteins that were differentially expressed in pooled serum samples from spontaneous preterm (cases [<34 weeks]) and term (controls) deliveries. Candidate protein expression was compared in individual serum samples between cases and controls matched by age and race groups, and clinical site. Protein expression was verified by Western blot in the placenta and fetal membranes from cases and controls
UR  - http://dx.doi.org/10.1016/j.ajog.2014.06.035
ER  -