Intratumoral CD3 and CD8 T-cell Densities Associated with Relapse-Free Survival in HCC. 

 -  2016 
<br/><br/> Copyright ©2016 American Association for Cancer Research.  Immune cells that infiltrate a tumor may be a prognostic factor for patients who have had surgically resected hepatocellular carcinoma (HCC). The density of intratumoral total (CD3(+)) and cytotoxic (CD8(+)) T lymphocytes was measured in the tumor interior and in the invasive margin of 65 stage I to IV HCC tissue specimens from a single cohort. Immune cell density in the interior and margin was converted to a binary score (0, low; 1, high), which was correlated with tumor recurrence and relapse-free survival (RFS). In addition, the expression of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) was correlated with the density of CD3(+) and CD8(+) cells and clinical outcome. High densities of both CD3(+) and CD8(+) T cells in both the interior and margin, along with corresponding Immunoscores, were significantly associated with a low rate of recurrence (P = 0.007) and a prolonged RFS (P = 0.002). In multivariate logistic regression models adjusted for vascular invasion and cellular differentiation, both CD3(+) and CD8(+) cell densities predicted recurrence, with odds ratios of 5.8 [95% confidence interval (CI), 1.6-21.8] for CD3(+) and 3.9 (95% CI, 1.1-14.1) for CD8(+) Positive PD-L1 staining was correlated with high CD3 and CD8 density (P = 0.024 and 0.005, respectively) and predicted a lower rate of recurrence (P = 0.034), as well as prolonged RFS (P = 0.029). Immunoscore and PD-L1 expression, therefore, are useful prognostic markers in patients with HCC who have undergone primary tumor resection. Cancer Immunol Res; 4(5); 419-30. ©2016 AACR.  
<br/><br/><br/>English 
<br/><br/><label>ISSN: </label>2326-6066 
<br/><br/><label>Subjects--Topical Terms: </label><br/>*Antigens, CD3/me [Metabolism]<br/>*Carcinoma, Hepatocellular/im [Immunology]<br/>*CD8-Positive T-Lymphocytes/im [Immunology]<br/>*Liver Neoplasms/im [Immunology]<br/>*Lymphocytes, Tumor-Infiltrating/im [Immunology]<br/>Adult<br/>Aged<br/>Aged, 80 and over<br/>Antigens, CD274/me [Metabolism]<br/>Biomarkers, Tumor/me [Metabolism]<br/>Carcinoma, Hepatocellular/di [Diagnosis]<br/>Carcinoma, Hepatocellular/pa [Pathology]<br/>Carcinoma, Hepatocellular/su [Surgery]<br/>Disease-Free Survival<br/>Female<br/>Follow-Up Studies<br/>Humans<br/>Kaplan-Meier Estimate<br/>Liver Neoplasms/di [Diagnosis]<br/>Liver Neoplasms/pa [Pathology]<br/>Liver Neoplasms/su [Surgery]<br/>Male<br/>Middle Aged<br/>Neoplasm Invasiveness<br/>Neoplasm Proteins/me [Metabolism]<br/>Neoplasm Staging<br/>Prognosis<br/>Recurrence<br/>Risk Factors<br/>T-Lymphocyte Subsets/im [Immunology]
<br/><br/><label>Subjects--Geographic Terms: </label><br/>MedStar Washington Hospital Center<br/>Washington Cancer Institute
<br/><br/><label>Index Terms--Occupation: </label><br/>Georgetown Transplant Institute<br/>Surgery/Transplantation
<br/><br/><label>Index Terms--Function: </label><br/>Journal Article