Intratumoral CD3 and CD8 T-cell Densities Associated with Relapse-Free Survival in HCC
Atkins, Michael
Fishbein, Thomas M
Satoskar, Rohit
text
xx
2016
monographic
en
Copyright ©2016 American Association for Cancer Research.
Immune cells that infiltrate a tumor may be a prognostic factor for patients who have had surgically resected hepatocellular carcinoma (HCC). The density of intratumoral total (CD3(+)) and cytotoxic (CD8(+)) T lymphocytes was measured in the tumor interior and in the invasive margin of 65 stage I to IV HCC tissue specimens from a single cohort. Immune cell density in the interior and margin was converted to a binary score (0, low; 1, high), which was correlated with tumor recurrence and relapse-free survival (RFS). In addition, the expression of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) was correlated with the density of CD3(+) and CD8(+) cells and clinical outcome. High densities of both CD3(+) and CD8(+) T cells in both the interior and margin, along with corresponding Immunoscores, were significantly associated with a low rate of recurrence (P = 0.007) and a prolonged RFS (P = 0.002). In multivariate logistic regression models adjusted for vascular invasion and cellular differentiation, both CD3(+) and CD8(+) cell densities predicted recurrence, with odds ratios of 5.8 [95% confidence interval (CI), 1.6-21.8] for CD3(+) and 3.9 (95% CI, 1.1-14.1) for CD8(+) Positive PD-L1 staining was correlated with high CD3 and CD8 density (P = 0.024 and 0.005, respectively) and predicted a lower rate of recurrence (P = 0.034), as well as prolonged RFS (P = 0.029). Immunoscore and PD-L1 expression, therefore, are useful prognostic markers in patients with HCC who have undergone primary tumor resection. Cancer Immunol Res; 4(5); 419-30. ©2016 AACR.
English
*Antigens, CD3/me [Metabolism]
*Carcinoma, Hepatocellular/im [Immunology]
*CD8-Positive T-Lymphocytes/im [Immunology]
*Liver Neoplasms/im [Immunology]
*Lymphocytes, Tumor-Infiltrating/im [Immunology]
Adult
Aged
Aged, 80 and over
Antigens, CD274/me [Metabolism]
Biomarkers, Tumor/me [Metabolism]
Carcinoma, Hepatocellular/di [Diagnosis]
Carcinoma, Hepatocellular/pa [Pathology]
Carcinoma, Hepatocellular/su [Surgery]
Disease-Free Survival
Female
Follow-Up Studies
Humans
Kaplan-Meier Estimate
Liver Neoplasms/di [Diagnosis]
Liver Neoplasms/pa [Pathology]
Liver Neoplasms/su [Surgery]
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins/me [Metabolism]
Neoplasm Staging
Prognosis
Recurrence
Risk Factors
T-Lymphocyte Subsets/im [Immunology]
MedStar Washington Hospital Center
Washington Cancer Institute
Georgetown Transplant Institute
Surgery/Transplantation
2326-6066
https://dx.doi.org/10.1158/2326-6066.CIR-15-0110
https://dx.doi.org/10.1158/2326-6066.CIR-15-0110
Ovid MEDLINE(R)
170303