Measuring thrombin activity in frozen brain tissue.

MedStar author(s):
Citation: Neuroreport. 28(17):1176-1179, 2017 Dec 06.PMID: 28915147Institution: MedStar Washington Hospital CenterDepartment: NeuroradiologyForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Brain/me [Metabolism] | *Frozen Sections | *Thrombin/me [Metabolism] | Animals | Female | Freezing | Functional Laterality | Male | Mice, Inbred C57BL | Molecular Probe Techniques | Sex CharacteristicsYear: 2017Local holdings: Available online from MWHC library: Dec 1996 - presentISSN:
  • 0959-4965
Name of journal: NeuroreportAbstract: Thrombin is a coagulation factor implicated in various pathological and physiological processes in the brain, exerting beneficial and deleterious effects in a concentration-dependent manner. Measurement of thrombin activity levels in pathological animal models is needed and in some cases, because of technical considerations, only frozen samples are available. In the current study, we used a quantitative method to evaluate thrombin activity in fresh and frozen brain sections of 43 male and female adult healthy mice. We stratified data per brain section, brain hemisphere, and mouse sex. We found lower thrombin activity in frozen sections compared with fresh sections, falling within levels considered central nervous system protective in previous studies. The results suggest that fresh section thrombin activity levels in healthy mice can be extrapolated from frozen brain sections. In addition, we found varying thrombin activity across the brain sections, with maximal activity in the olfactory system and hippocampus-containing sections. Thrombin activity did not vary between males and females, or between the right and the left hemispheres, in a statistically significantly manner.All authors: Chapman J, Golderman V, Harnof S, Reuveni G, Rosman Y, Shavit-Stein E, Shrot SOriginally published: Neuroreport. , 2017 Sep 14Fiscal year: FY2018Digital Object Identifier: Date added to catalog: 2017-09-22
Holdings
Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 28915147 Available 28915147

Available online from MWHC library: Dec 1996 - present

Thrombin is a coagulation factor implicated in various pathological and physiological processes in the brain, exerting beneficial and deleterious effects in a concentration-dependent manner. Measurement of thrombin activity levels in pathological animal models is needed and in some cases, because of technical considerations, only frozen samples are available. In the current study, we used a quantitative method to evaluate thrombin activity in fresh and frozen brain sections of 43 male and female adult healthy mice. We stratified data per brain section, brain hemisphere, and mouse sex. We found lower thrombin activity in frozen sections compared with fresh sections, falling within levels considered central nervous system protective in previous studies. The results suggest that fresh section thrombin activity levels in healthy mice can be extrapolated from frozen brain sections. In addition, we found varying thrombin activity across the brain sections, with maximal activity in the olfactory system and hippocampus-containing sections. Thrombin activity did not vary between males and females, or between the right and the left hemispheres, in a statistically significantly manner.

English

Powered by Koha