Quantitative analysis of corneal stromal riboflavin concentration without epithelial removal.

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Citation: Journal of Cataract & Refractive Surgery. 44(2):237-242, 2018 Feb.PMID: 29526339Institution: MedStar Washington Hospital CenterDepartment: OphthalmologyForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Corneal Stroma/me [Metabolism] | *Epithelium, Corneal/su [Surgery] | *Photosensitizing Agents/pk [Pharmacokinetics] | *Riboflavin/pk [Pharmacokinetics] | Animals | Chromatography, Liquid | Cross-Linking Reagents/pk [Pharmacokinetics] | Debridement | Mass Spectrometry | RabbitsYear: 2018Local holdings: Available online through MWHC library: 1999 - 2010, Available in print through MWHC library:1999-2007ISSN:
  • 0886-3350
Name of journal: Journal of cataract and refractive surgeryAbstract: CONCLUSIONS: The system used in Group 1 produced corneal riboflavin concentrations that were theoretically adequate for effective transepithelial CXL (>=15 mug/g), while the system in Group 2 did not. Slitlamp grading successfully estimated the corneal riboflavin concentration and can be used to ensure an adequate concentration of riboflavin in the cornea for transepithelial CXL.Copyright (c) 2018 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.DESIGN: Experimental study.METHODS: The stromal riboflavin concentration of 2 transepithelial CXL systems was compared in rabbit eyes in vivo. The systems were the CXLO (Group 1) and Paracel/Vibex Xtra, containing TRIS and ethylenediaminetetraacetic acid and an isotonic solution of riboflavin 0.25%, (Group 2). Manufacturers' Instructions For Use were followed. The intensity of riboflavin fluorescence by slitlamp observation 10, 15, and 20 minutes after instillation was graded on a scale of 0 to 5. The animals were humanely killed and the corneal stromal samples analyzed with liquid chromatography and mass spectrometry.PURPOSE: To compare the corneal stromal riboflavin concentration and distribution using 2 transepithelial corneal crosslinking (CXL) systems.RESULTS: The mean riboflavin fluorescence intensity grades in Group 1 (4 eyes) were 3.8, 4.8, and 4.8 at 10, 15, and 20 minutes, respectively. The mean grades in Group 2 (3 eyes) were 2.0, 2.3, and 2.0, respectively. The riboflavin distribution was uniform in Group 1 but not in Group 2. The mean riboflavin concentration by liquid chromatography and mass spectrometry was 27.0 mug/g stromal tissue in Group 1 and 6.7 mug/g in Group 2. A stromal riboflavin concentration theoretically adequate for CXL, 15 mug/g, was achieved in all eyes in Group 1 and no eyes in Group 2. Slitlamp grading correlated well with liquid chromatography and mass spectrometry concentration (R<sup>2</sup> = 0.940).SETTING: Absorption Systems, San Diego, California, USA.All authors: Gum GG, Rubinfeld RS, Stulting RD, Talamo JHFiscal year: FY2018Digital Object Identifier: Date added to catalog: 2018-04-19
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Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 29526339 Available 29526339

Available online through MWHC library: 1999 - 2010, Available in print through MWHC library:1999-2007

CONCLUSIONS: The system used in Group 1 produced corneal riboflavin concentrations that were theoretically adequate for effective transepithelial CXL (>=15 mug/g), while the system in Group 2 did not. Slitlamp grading successfully estimated the corneal riboflavin concentration and can be used to ensure an adequate concentration of riboflavin in the cornea for transepithelial CXL.

Copyright (c) 2018 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.

DESIGN: Experimental study.

METHODS: The stromal riboflavin concentration of 2 transepithelial CXL systems was compared in rabbit eyes in vivo. The systems were the CXLO (Group 1) and Paracel/Vibex Xtra, containing TRIS and ethylenediaminetetraacetic acid and an isotonic solution of riboflavin 0.25%, (Group 2). Manufacturers' Instructions For Use were followed. The intensity of riboflavin fluorescence by slitlamp observation 10, 15, and 20 minutes after instillation was graded on a scale of 0 to 5. The animals were humanely killed and the corneal stromal samples analyzed with liquid chromatography and mass spectrometry.

PURPOSE: To compare the corneal stromal riboflavin concentration and distribution using 2 transepithelial corneal crosslinking (CXL) systems.

RESULTS: The mean riboflavin fluorescence intensity grades in Group 1 (4 eyes) were 3.8, 4.8, and 4.8 at 10, 15, and 20 minutes, respectively. The mean grades in Group 2 (3 eyes) were 2.0, 2.3, and 2.0, respectively. The riboflavin distribution was uniform in Group 1 but not in Group 2. The mean riboflavin concentration by liquid chromatography and mass spectrometry was 27.0 mug/g stromal tissue in Group 1 and 6.7 mug/g in Group 2. A stromal riboflavin concentration theoretically adequate for CXL, 15 mug/g, was achieved in all eyes in Group 1 and no eyes in Group 2. Slitlamp grading correlated well with liquid chromatography and mass spectrometry concentration (R<sup>2</sup> = 0.940).

SETTING: Absorption Systems, San Diego, California, USA.

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