Efficacy of a mesenchymal stem cell loaded surgical mesh for tendon repair in rats.
Citation: J. transl. med.. 12:110, 2014.Journal of Translational Medicine. 12:110, 2014.PMID: 24884819Institution: MedStar Union Memorial HospitalDepartment: Orthopaedic SurgeryForm of publication: Journal ArticleMedline article type(s): Journal Article | Research Support, Non-U.S. Gov'tSubject headings: *Mesenchymal Stromal Cells/cy [Cytology] | *Surgical Mesh | *Tendon Injuries/th [Therapy] | *Tissue Scaffolds | Animals | Humans | Male | Rats | Rats, Sprague-DawleyYear: 2014Local holdings: Available online through MWHC library: 2003 - presentISSN:- 1479-5876
Item type | Current library | Collection | Call number | Status | Date due | Barcode |
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Journal Article | MedStar Authors Catalog | Article | 24884819 | Available | 24884819 |
Available online through MWHC library: 2003 - present
CONCLUSION: The MSC-loaded mesh enhanced early tendon healing, particularly the quality of collagen bundles. Application of the MSC-loaded mesh, as a new device and MSC delivery vehicle, may benefit to early functional recovery of the ruptured tendon.
METHODS: The MSC-loaded mesh composed of a piece of conventional surgical mesh and a layer of scaffold, which supported MSC-embedded alginate gel. A 3-mm defect was surgically created at the Achilles tendon-gastrocnemius/soleus junction in 30 rats. The tendon defects were repaired with either 1) MSC-loaded mesh; or 2) surgical mesh only; or 3) routine surgical suture. Repaired tendons were harvested at days 6 and 14 for histology, which was scored on the bases of collagen organization, vascularity and cellularity, and immunohistochemisty of types I and III collagen.
OBJECTIVES: The purpose of this study was to investigate the efficacy of a composite surgical mesh for delivery of mesenchymal stem cells (MSCs) in tendon repair.
RESULTS: In comparison with the other two repair types, at day 6, the MSC-loaded mesh significantly improved the quality of the repaired tendons with dense and parallel collagen bundles, reduced vascularity and increased type I collagen. At day 14, the MSC-loaded mesh repaired tendons had better collagen formation and organization.
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