Functionally compromised synovium-derived mesenchymal stem cells in Charcot neuroarthropathy.

MedStar author(s):
Citation: Experimental & Molecular Pathology. 104(1):82-88, 2018 FebPMID: 29353045Institution: MedStar Union Memorial HospitalDepartment: Orthobiologic Laboratory | Orthopaedic SurgeryForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Arthropathy, Neurogenic/pa [Pathology] | *Mesenchymal Stromal Cells/cy [Cytology] | *Synovial Membrane/cy [Cytology] | Adipogenesis/ph [Physiology] | Cell Differentiation/ph [Physiology] | Cells, Cultured | Chondrogenesis/ph [Physiology] | Female | Foot/pa [Pathology] | Humans | Male | Mesenchymal Stromal Cells/pa [Pathology] | Middle Aged | Osteogenesis/ph [Physiology] | Synovial Membrane/pa [Pathology]Year: 2018ISSN:
  • 0014-4800
Name of journal: Experimental and molecular pathologyAbstract: Charcot neuroarthropathy (CNA) often presents as a diabetic foot complication. The role of synovial mesenchymal stem cells (syn-MSCs) in the pathogenesis of CNA is unclear. Synovial samples were collected, for isolation of syn-MSCs, from diabetic patients with CNA (n=7) and non-diabetic patients with intra-articular fracture or normal joints (non-CNA; n=7) during foot surgery. The syn-MSCs in the CNA and non-CNA groups were characterized comparatively. The average number of colonies formed in the CNA group was 6+/-3.5 per half plate (10mm in diameter), while it was 43+/-21.6 in the non-CNA group (p<0.05). The average size (pixels) of the colonies in the CNA group was smaller than that in the non-CNA group. When the colonies were stratified into high-, medium- and low-density subgroups, colonies in the high-density subgroup of the CNA group were reduced in density. Expression of PPAR-gamma, RUNX2, Sox9 and type II collagen by syn-MSCs in the CNA group was decreased during adipogenic, osteogenic and chondrogenic differentiation as compared with the non-CNA group. In conclusion, syn-MSCs in CNA joints were reduced in number, with declined differentiation potentials. The high-density subpopulation of the syn-MSCs was particularly affected by the pathology of CNA.Copyright (c) 2018 Elsevier Inc. All rights reserved.All authors: Cho Y, Mitchell R, Molligan J, Rooney S, Schon L, Zhang ZFiscal year: FY2018Digital Object Identifier: Date added to catalog: 2018-02-20
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Journal Article MedStar Authors Catalog Article 29353045 Available 29353045

Charcot neuroarthropathy (CNA) often presents as a diabetic foot complication. The role of synovial mesenchymal stem cells (syn-MSCs) in the pathogenesis of CNA is unclear. Synovial samples were collected, for isolation of syn-MSCs, from diabetic patients with CNA (n=7) and non-diabetic patients with intra-articular fracture or normal joints (non-CNA; n=7) during foot surgery. The syn-MSCs in the CNA and non-CNA groups were characterized comparatively. The average number of colonies formed in the CNA group was 6+/-3.5 per half plate (10mm in diameter), while it was 43+/-21.6 in the non-CNA group (p<0.05). The average size (pixels) of the colonies in the CNA group was smaller than that in the non-CNA group. When the colonies were stratified into high-, medium- and low-density subgroups, colonies in the high-density subgroup of the CNA group were reduced in density. Expression of PPAR-gamma, RUNX2, Sox9 and type II collagen by syn-MSCs in the CNA group was decreased during adipogenic, osteogenic and chondrogenic differentiation as compared with the non-CNA group. In conclusion, syn-MSCs in CNA joints were reduced in number, with declined differentiation potentials. The high-density subpopulation of the syn-MSCs was particularly affected by the pathology of CNA.

Copyright (c) 2018 Elsevier Inc. All rights reserved.

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