000			02651nam a22003617a 4500
008			201229s20192019 xxu||||| |||| 00| 0 eng d
022			_a1937-5719
040			_aOvid MEDLINE(R)
099			_a30858901
245			_aPhotobiomodulation Elicits a Differential Cytokine Response in a Cultured Analogue of Human Skin.
251			_aEplasty [Electronic Resource]. 19:e3, 2019.
252			_aEplasty. 19:e3, 2019.
253			_aEplasty
260			_c2019
260			_fFY2019
265			_sepublish
266			_d2020-12-29
520			_aBackground: The study of photobiomodulation in wound healing is encumbered by limited wound study models. The aim of this study was to investigate the efficacy of a 3-dimensional dermal tissue culture model as a cost-saving alternative to conventional photobiomodulation study techniques. Methods: Nine dermal analogue tissue cultures were treated for 2 days with sham or 660-nm wavelength of light at either 1.5 or 3 mW/cm2 of energy. Tissue cytokine mRNA production was assessed by real-time reverse transcription-polymerase chain reaction, and tissue and supernatant protein were evaluated by immunofluorescence, enzyme-linked immunosorbent assay, and Western blot. Results: Photobiomodulation with 660-nm wavelength light induced transcription of IL-1beta and IL-6 mRNA and decreased that of IL-8. Tissue protein content of IL-6 and IL-8 was unchanged, whereas supernatant protein content of IL-8 was significantly increased (P = .023) by 1.5 mW/cm2 treatment. To describe the localization of cytokines between tissue and supernatant, the relative diffusion of each was calculated and found to be 15-fold higher for IL-6 than for IL-8 despite an overall higher concentration of IL-8 in the tissue. Conclusion: In this study, photobiomodulation elicited mRNA and protein changes quantifiable in both the tissue and supernatant. In addition, the use of this advanced culture model allowed for histological assessment and the comparison of "local" versus "circulatory" responses between the tissue and supernatant, respectively.
546			_aEnglish
650			_aIN PROCESS -- NOT YET INDEXED
651			_aMedStar Health Research Institute
651			_aMedStar Washington Hospital Center
656			_aFirefighters' Burn and Surgical Research Laboratory
656			_aSurgery/Burn Services
657			_aJournal Article
700			_aArdanuy, Jeremy G
700			_aCarney, Bonnie C
700			_aMoffatt, Lauren T
700			_aPrindeze, Nicholas J
700			_aShupp, Jeffrey W
790			_aArdanuy JG, Carney BC, Moffatt LT, Prindeze NJ, Shupp JW
942			_cART _dArticle
999			_c4180 _d4180