000			03092nam a22004817a 4500
008			201006s20202020 xxu||||| |||| 00| 0 eng d
022			_a2045-2322
024			_a10.1038/s41598-020-72511-8 [doi]
024			_a10.1038/s41598-020-72511-8 [pii]
040			_aOvid MEDLINE(R)
099			_a32973266
245			_aBurn resuscitation strategy influences the gut microbiota-liver axis in swine.
251			_aScientific Reports. 10(1):15655, 2020 09 24.
252			_aSci. rep.. 10(1):15655, 2020 09 24.
252			_zSci. rep.. 10(1):15655, 2020 Sep 24.
253			_aScientific reports
260			_c2020
260			_fFY2021
265			_sepublish
265			_sepublish
266			_d2020-10-06
268			_aScientific Reports. 10(1):15655, 2020 Sep 24.
520			_aFluid resuscitation improves clinical outcomes of burn patients; however, its execution in resource-poor environments may have to be amended with limited-volume strategies. Liver dysfunction is common in burn patients and gut dysbiosis is an understudied aspect of burn sequelae. Here, the swine gut microbiota and liver transcripts were investigated to determine the impact of standard-of-care modified Brooke (MB), limited-volume colloid (LV-Co), and limited-volume crystalloid (LV-Cr) resuscitation on the gut microbiota, and to evaluate its' potential relationship with liver dysfunction. Independent of resuscitation strategy, bacterial diversity was reduced 24 h post-injury, and remained perturbed at 48 h. Changes in community structure were most pronounced with LV-Co, and correlated with biomarkers of hepatocellular damage. Hierarchical clustering revealed a group of samples that was suggestive of dysbiosis, and LV-Co increased the risk of association with this group. Compared with MB, LV-Co and LV-Cr significantly altered cellular stress and ATP pathways, and gene expression of these perturbed pathways was correlated with major dysbiosis-associated bacteria. Taken together, LV-Co resuscitation exacerbated the loss of bacterial diversity and increased the risk of dysbiosis. Moreover, we present evidence of a linkage between liver (dys)function and the gut microbiota in the acute setting of burn injury.
546			_aEnglish
650			_a*Burns/mi [Microbiology]
650			_a*Burns/th [Therapy]
650			_a*Gastrointestinal Microbiome
650			_a*Liver/pp [Physiopathology]
650			_aAnimals
650			_aBurns/me [Metabolism]
650			_aBurns/pp [Physiopathology]
650			_aDysbiosis/co [Complications]
650			_aGene Expression Regulation
650			_aLiver/me [Metabolism]
650			_aSwine
651			_aMedStar Health Research Institute
656			_aFirefighters' Burn and Surgical Research Laboratory
657			_aJournal Article
700			_aShupp, Jeffrey W
790			_aBurmeister DM, Bynum JA, Chung KK, Dubick MA, Gomez BI, Granados JC, Muraoka WT, Nicholson SE, Shupp JW
856			_uhttps://dx.doi.org/10.1038/s41598-020-72511-8 _zhttps://dx.doi.org/10.1038/s41598-020-72511-8
942			_cART _dArticle
999			_c5614 _d5614