000			03189nam a22003497a 4500
008			201229s20202020 xxu||||| |||| 00| 0 eng d
022			_a2072-1439
040			_aOvid MEDLINE(R)
099			_a27606077
245			_aTissue requirements in lung cancer diagnosis for tumor heterogeneity, mutational analysis and targeted therapies: initial experience with intra-operative Frozen Section Evaluation (FROSE) in bronchoscopic biopsies.
251			_aJournal of Thoracic Disease. 8(Suppl 6):S488-93, 2016 Jul.
252			_aJ. thorac. dis.. 8(Suppl 6):S488-93, 2016 Jul.
253			_aJournal of thoracic disease
260			_c2016
260			_fFY2017
265			_sppublish
266			_d2020-12-29
520			_aBACKGROUND: Recent advances in lung cancer treatment have changed the requirement for the amount and quality of biopsy specimens needed to characterize the tumor and select the best treatment. One adjunct to guide the bronchoscopist on the quality and quantity of specimens during bronchoscopic biopsies for the diagnosis of lung cancer is rapid on-site evaluation (ROSE) of cytological specimens. This technique has been shown to add to the diagnostic yield of bronchoscopy when obtaining adequate specimens for molecular profiling in lung cancer. ROSE is not available at all medical centers. We describe our initial experience using intra-procedural Frozen Section Evaluation (FROSE) of bronchoscopic biopsy specimens as an alternative to ROSE.
520			_aCONCLUSIONS: In medical centers where ROSE may not be available, the use of FROSE by the local pathologist can be an effective technique to obtain adequate tissue and cytological samples for the diagnosis and molecular profiling of lung cancers. Further prospective study in bronchoscopic tissue sampling techniques to obtain the optimum quantity and quality of samples for molecular profiling of lung cancers for targeted treatments is needed.
520			_aMETHODS: A retrospective analysis of all interventional pulmonology cases using FROSE between February and July 2015 was performed. Results analyzed to evaluate the success in obtaining adequate specimens for molecular profiling.
520			_aRESULTS: A total of 88 interventional pulmonology cases employing a frozen section in at least one site were identified. In 94.3% of cases, a definitive diagnosis of benign or malignant was made. The concordance of frozen section diagnoses of benign or malignant was 100% with final diagnoses. Thirteen of the eighty-eight cases were ultimately sent for molecular analysis. Of these, twelve of thirteen (92.3%) cases were adequate to perform all ordered molecular testing. In all cases there was sufficient tissue to perform EGFR and ALK testing.
546			_aEnglish
650			_aIN PROCESS -- NOT YET INDEXED
651			_aMedStar Franklin Square Medical Center
656			_aPulmonary and Critical Care Medicine
657			_aJournal Article
700			_aIding, Jeffrey S
700			_aKrimsky, William
790			_aBrowning R, Iding JS, Krimsky W
856			_uhttps://dx.doi.org/10.21037/jtd.2016.03.17 _zhttps://dx.doi.org/10.21037/jtd.2016.03.17
942			_cART _dArticle
999			_c5717 _d5717