000			03890nam a22006257a 4500
008			220124s20212021 xxu||||| |||| 00| 0 eng d
022			_a0022-4804
024			_a10.1016/j.jss.2021.05.010 [doi]
024			_aS0022-4804(21)00317-6 [pii]
040			_aOvid MEDLINE(R)
099			_a34153561
245			_aA Pilot Study of Negative Pressure Therapy with Autologous Skin Cell Suspensions in a Porcine Model.
251			_aJournal of Surgical Research. 267:182-196, 2021 11.
252			_aJ Surg Res. 267:182-196, 2021 11.
252			_zJ Surg Res. 267:182-196, 2021 Nov.
253			_aThe Journal of surgical research
260			_c2021
260			_fFY2022
260			_p2021 Nov
265			_sppublish
266			_d2021-07-19
268			_aJournal of Surgical Research. 267:182-196, 2021 Nov.
520			_aBACKGROUND: Negative pressure wound therapy (NPWT) is an option for securing meshed split thickness skin grafts (mSTSGs) after burn excision to optimize skin graft adherence. Recently, the use of autologous skin cell suspension (ASCS) has been approved for use in the treatment of burn injuries in conjunction with mSTSGs.To date, limited data exists regarding the impact of NPWT on healing outcomes when the cellular suspension is utilized. It was hypothesized that NPWT would not negatively impact wound healing of ASCS+mSTSG.
520			_aCONCLUSIONS: These data suggest the positive attributes of the cellular suspension delivered are retained following the application of negative pressure. Re-epithelialization, revascularization, and repigmentation are not adversely impacted. The use of NPWT may be considered as an option when using ASCS with mSTSGs for the treatment of full-thickness burns. Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
520			_aMATERIALS AND METHODS: A burn, excision, mSTSG, ASCS +/- NPWT model was used. Two Duroc pigs were utilized in this experiment, each with 2 sets of paired burns. Four wounds received mSTSG+ASCS+NPWT through post-operative day 3, and 4 wounds received mSTSG+ACSC+ traditional ASCS dressings. Cellular viability was characterized prior to spraying. Percent re-epithelialization, graft-adherence, pigmentation, elasticity, and blood perfusion and blood vessel density were assessed at multiple time points through 2 weeks.
520			_aRESULTS: All wounds healed within 14 days with minimal scar pathology and no significant differences in percent re-epithelialization between NPWT, and non-NPWT wounds were observed. Additionally, no differences were detected for pigmentation, perfusion, or blood vessel density. NPWT treated wounds had less graft loss and improved elasticity, with elasticity being statistically different.
546			_aEnglish
650			_a*Burns
650			_a*Negative-Pressure Wound Therapy
650			_aAnimals
650			_aBurns/pa [Pathology]
650			_aPilot Projects
650			_aSkin Transplantation
650			_aSkin/pa [Pathology]
650			_aSuspensions
650			_aSwine
651			_aMedStar Health Research Institute
651			_aMedStar Washington Hospital Center
656			_aBurn Research Fellowship
656			_aFirefighters' Burn and Surgical Research Laboratory
656			_aSurgery/Burn Services
657			_aJournal Article
700			_aCarney, Bonnie C
700			_aKeyloun, John
700			_aKirkpatrick, Liam D
700			_aMoffatt, Lauren T
700			_aNisar, Saira
700			_aOliver, Mary A
700			_aPrindeze, Nicholas
700			_aShupp, Jeffrey W
700			_aTravis, Taryn E
790			_aCarney BC, Keyloun JW, Kirkpatrick LD, Moffatt LT, Nisar S, Oliver MA, Prindeze NJ, Shupp JW, Travis TE
856			_uhttps://dx.doi.org/10.1016/j.jss.2021.05.010 _zhttps://dx.doi.org/10.1016/j.jss.2021.05.010
942			_cART _dArticle
999			_c6584 _d6584