Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis.

MedStar author(s):
Citation: Foot & Ankle International. 35(12):1241-9, 2014 Dec.Foot Ankle Int. 35(12):1241-9, 2014 Dec.PMID: 25201328Institution: MedStar Union Memorial HospitalDepartment: Orthopaedic SurgeryForm of publication: Journal ArticleMedline article type(s): Comparative Study | Journal Article | Research Support, Non-U.S. Gov'tSubject headings: *Ankle Joint/me [Metabolism] | *Arthritis/di [Diagnosis] | *Cytokines/me [Metabolism] | *Metabolome/ph [Physiology] | *Synovial Fluid/me [Metabolism] | *Wounds and Injuries/co [Complications] | Adult | Aged | Ankle Joint/pp [Physiopathology] | Arthritis, Rheumatoid/di [Diagnosis] | Arthritis, Rheumatoid/me [Metabolism] | Arthritis/et [Etiology] | Arthritis/ra [Radiography] | Biomarkers/an [Analysis] | Biomarkers/me [Metabolism] | Case-Control Studies | Diagnosis, Differential | Enzyme-Linked Immunosorbent Assay | Female | Humans | Inflammation Mediators/me [Metabolism] | Male | Mass Spectrometry | Middle Aged | Osteoarthritis/di [Diagnosis] | Osteoarthritis/me [Metabolism] | Reference Values | Sampling Studies | Sensitivity and Specificity | Wounds and Injuries/di [Diagnosis]Year: 2014Local holdings: Available online from MWHC library: 1999 - present, Available in print through MWHC library: 1999 - 2006ISSN:
  • 1071-1007
Name of journal: Foot & ankle internationalAbstract: BACKGROUND: There is a paucity of research on posttraumatic ankle arthritis (PTAA). We aimed to identify synovial fluid PTAA biomarkers using cytokine analysis and metabolic profiling.CLINICAL RELEVANCE: Several of these entities have previously been implicated in rheumatoid arthritis and osteoarthritis of the knee, but many could potentially be used as novel biomarkers of PTAA. Most importantly, the findings suggest that metabolites could be used to distinguish synovial fluid from patients with PTAA.Copyright © The Author(s) 2014.CONCLUSION: This study identified inflammatory cytokines and metabolites present in the synovial fluid of PTAA.METHODS: Ankle joint synovial fluid was obtained from 20 patients with PTAA and 20 patients with no ankle pain and no radiographic evidence of ankle arthritis (control group). Synovial fluid samples were analyzed for IFN-gamma, TNF-alpha, MIP-1beta, MCP-1, IL-1beta, IL-1Ra, IL-4, IL-6, IL-8, IL-10, IL-13, and IL-15 using ELISA and for more than 3000 metabolites using liquid and gas chromatography with mass spectroscopy. To compare presence of cytokines and metabolites between groups, t tests were used. Random forest analysis was performed on metabolites to determine whether control and PTAA samples could be differentiated based on metabolic profile.RESULTS: IL-1Ra, IL-6, IL-8, IL-10, IL-15, and MCP-1 were significantly elevated in the PTAA group. In addition, 107 metabolites in the PTAA group were significantly altered, including derangement in amino acid, carbohydrate, lipid, and energy metabolism, extracellular matrix turnover, and collagen degradation. Random forest analysis yielded a predictive accuracy of 90% when using the metabolic profiles to distinguish between control and PTAA samples.All authors: Adams SB Jr, Guyton GP, Jones LC, Miller SD, Nettles DL, Schon LCFiscal year: FY2015Digital Object Identifier: Date added to catalog: 2016-07-15
Holdings
Item type Current library Collection Call number Status Date due Barcode
Journal Article MedStar Authors Catalog Article 25201328 Available 25201328

Available online from MWHC library: 1999 - present, Available in print through MWHC library: 1999 - 2006

BACKGROUND: There is a paucity of research on posttraumatic ankle arthritis (PTAA). We aimed to identify synovial fluid PTAA biomarkers using cytokine analysis and metabolic profiling.

CLINICAL RELEVANCE: Several of these entities have previously been implicated in rheumatoid arthritis and osteoarthritis of the knee, but many could potentially be used as novel biomarkers of PTAA. Most importantly, the findings suggest that metabolites could be used to distinguish synovial fluid from patients with PTAA.Copyright © The Author(s) 2014.

CONCLUSION: This study identified inflammatory cytokines and metabolites present in the synovial fluid of PTAA.

METHODS: Ankle joint synovial fluid was obtained from 20 patients with PTAA and 20 patients with no ankle pain and no radiographic evidence of ankle arthritis (control group). Synovial fluid samples were analyzed for IFN-gamma, TNF-alpha, MIP-1beta, MCP-1, IL-1beta, IL-1Ra, IL-4, IL-6, IL-8, IL-10, IL-13, and IL-15 using ELISA and for more than 3000 metabolites using liquid and gas chromatography with mass spectroscopy. To compare presence of cytokines and metabolites between groups, t tests were used. Random forest analysis was performed on metabolites to determine whether control and PTAA samples could be differentiated based on metabolic profile.

RESULTS: IL-1Ra, IL-6, IL-8, IL-10, IL-15, and MCP-1 were significantly elevated in the PTAA group. In addition, 107 metabolites in the PTAA group were significantly altered, including derangement in amino acid, carbohydrate, lipid, and energy metabolism, extracellular matrix turnover, and collagen degradation. Random forest analysis yielded a predictive accuracy of 90% when using the metabolic profiles to distinguish between control and PTAA samples.

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