TY  - BOOK
AU  - Jeng, James C
AU  - Jo, Daniel Y
AU  - Jordan, Marion H
AU  - Leto, Ellen J
AU  - Moffatt, Lauren T
AU  - Randad, Pranay R
AU  - Shupp, Jeffrey W
TI  - Examination of local and systemic in vivo responses to electrical injury using an electrical burn delivery system
SN  - 1559-047X
PY  - 2012///
KW  - *Burns, Electric/ge [Genetics]
KW  - *Burns, Electric/me [Metabolism]
KW  - *Cytokines/me [Metabolism]
KW  - *RNA, Messenger/an [Analysis]
KW  - Animals
KW  - Biological Markers/me [Metabolism]
KW  - Blood Chemical Analysis
KW  - Burns, Electric/pa [Pathology]
KW  - Cytokines/an [Analysis]
KW  - Disease Models, Animal
KW  - Down-Regulation
KW  - Gene Expression Regulation
KW  - Immunohistochemistry
KW  - Injury Severity Score
KW  - Laser-Doppler Flowmetry
KW  - Male
KW  - Physical Examination
KW  - Polymerase Chain Reaction/mt [Methods]
KW  - Random Allocation
KW  - Rats
KW  - Rats, Sprague-Dawley
KW  - Risk Factors
KW  - Wound Healing/ph [Physiology]
KW  - MedStar Washington Hospital Center
KW  - Surgery/Burn Services
KW  - Comparative Study
KW  - Journal Article
KW  - Research Support, Non-U.S. Gov't
N1  - Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present
N2  - Electrical injuries are devastating and are difficult to manage due to the complexity of the tissue damage and physiological impacts. A paucity of literature exists which describes models for electrical injury. To date, those models have been used primarily to demonstrate thermal and morphological effects at the points of contact. Creating a more representative model for human injury and further elucidating the physics and pathophysiology of this unique form of tissue injury could be helpful in designing stage-appropriate therapy and improving limb salvage. An electrical burn delivery system was developed to accurately and reliably deliver electrical current at varying exposure times. A series of Sprague-Dawley rats were anesthetized and subjected to injury with 1000 V of direct current at incremental exposure times (2-20 seconds). Whole blood and plasma were obtained immediately before shock, immediately postinjury, and then hourly for 3 hours. Laser Doppler images of tissue adjacent to the entrance and exit wounds were obtained at the outlined time points to provide information on tissue perfusion. The electrical exposure was nonlethal in all animals. The size and the depth of contact injury increased in proportion to the exposure times and were reproducible. Skin adjacent to injury (both entrance and exit sites) exhibited marked edema within 30 minutes. In adjacent skin of upper extremity wounds, mean perfusion units increased immediately postinjury and then gradually decreased in proportion to the severity of the injuries. In the lower extremity, this phenomenon was only observed for short contact times, while longer contact times had marked malperfusion throughout. In the plasma, interleukin-10 and vascular endothelial growth factor levels were found to be augmented by injury. Systemic transcriptome analysis revealed promising information about signal networks involved in dermatological, connective tissue, and neurological pathophysiological processes. A reliable and reproducible in vivo model has been developed for characterizing the pathophysiology of high-tension electrical injury. 130912s in perfusion were observed near and between entrance and exit wounds that appear consistent with injury severity. Further studies are underway to correlate differential mRNA expression with injury severity
UR  - http://dx.doi.org/10.1097/BCR.0b013e3182373a50
ER  -