TY  - BOOK
AU  - Alkhalil, Abdulnaser
AU  - Carney, Bonnie C
AU  - Liu, Zekun
AU  - Moffatt, Lauren T
AU  - Shupp, Jeffrey W
AU  - Travis, Taryn E
TI  - Elastin Is Differentially Regulated by Pressure Therapy in a Porcine Model of Hypertrophic Scar
SN  - 1559-047X
PY  - 2017///
KW  - *Cicatrix, Hypertrophic/me [Metabolism]
KW  - *Cicatrix, Hypertrophic/th [Therapy]
KW  - *Elastin/me [Metabolism]
KW  - *Pressure
KW  - *Wounds, Penetrating/th [Therapy]
KW  - Animals
KW  - Cicatrix, Hypertrophic/pa [Pathology]
KW  - Disease Models, Animal
KW  - Male
KW  - Pliability
KW  - Swine
KW  - Wounds, Penetrating/co [Complications]
KW  - Wounds, Penetrating/pa [Pathology]
KW  - MedStar Health Research Institute
KW  - MedStar Washington Hospital Center
KW  - Surgery/Burn Services
KW  - Journal Article
N1  - Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present
N2  - Beneficial effects of pressure therapy for hypertrophic scars have been reported, but the mechanisms of action are not fully understood. This study evaluated elastin and its contribution to scar pliability. The relationship between changes in Vancouver Scar Scale (VSS) scores of pressure-treated scars and differential regulation of elastin was assessed. Hypertrophic scars were created and assessed weekly using VSS and biopsy procurement. Pressure treatment began on day 70 postinjury. Treated scars were compared with untreated shams. Treatment lasted 2 weeks, through day 84, and scars were assessed weekly through day 126. Transcript and protein levels of elastin were quantified. Pressure treatment resulted in lower VSS scores compared with sham-treated scars. Pliability (VSSP) was a key contributor to this difference. At day 70 pretreatment, VSSP = 2. Without treatment, sham-treated scars became less pliable, while pressure-treated scars became more pliable. The percentage of elastin in scars at day 70 was higher than in uninjured skin. Following treatment, the percentage of elastin increased and continued to increase through day 126. Untreated sham scars did not show a similar increase. Quantification of Verhoeff-Van Gieson staining corroborated the findings and immunofluorescence revealed the alignment of elastin fibers. Pressure treatment results in increased protein level expression of elastin compared with sham-untreated scars. These findings further characterize the extracellular matrix's response to the application of pressure as a scar treatment, which will contribute to the refinement of rehabilitation practices and ultimately improvements in functional and psychosocial outcomes for patients
UR  - https://dx.doi.org/10.1097/BCR.0000000000000413
ER  -