TY  - BOOK
AU  - Adams, Samuel B Jr
AU  - Guyton, Gregory P
AU  - Miller, Stuart D
AU  - Schon, Lew C
TI  - Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis
SN  - 1071-1007
PY  - 2014///
KW  - *Ankle Joint/me [Metabolism]
KW  - *Arthritis/di [Diagnosis]
KW  - *Cytokines/me [Metabolism]
KW  - *Metabolome/ph [Physiology]
KW  - *Synovial Fluid/me [Metabolism]
KW  - *Wounds and Injuries/co [Complications]
KW  - Adult
KW  - Aged
KW  - Ankle Joint/pp [Physiopathology]
KW  - Arthritis, Rheumatoid/di [Diagnosis]
KW  - Arthritis, Rheumatoid/me [Metabolism]
KW  - Arthritis/et [Etiology]
KW  - Arthritis/ra [Radiography]
KW  - Biomarkers/an [Analysis]
KW  - Biomarkers/me [Metabolism]
KW  - Case-Control Studies
KW  - Diagnosis, Differential
KW  - Enzyme-Linked Immunosorbent Assay
KW  - Female
KW  - Humans
KW  - Inflammation Mediators/me [Metabolism]
KW  - Male
KW  - Mass Spectrometry
KW  - Middle Aged
KW  - Osteoarthritis/di [Diagnosis]
KW  - Osteoarthritis/me [Metabolism]
KW  - Reference Values
KW  - Sampling Studies
KW  - Sensitivity and Specificity
KW  - Wounds and Injuries/di [Diagnosis]
KW  - MedStar Union Memorial Hospital
KW  - Orthopaedic Surgery
KW  - Comparative Study
KW  - Journal Article
KW  - Research Support, Non-U.S. Gov't
N1  - Available online from MWHC library: 1999 - present, Available in print through MWHC library: 1999 - 2006
N2  - BACKGROUND: There is a paucity of research on posttraumatic ankle arthritis (PTAA). We aimed to identify synovial fluid PTAA biomarkers using cytokine analysis and metabolic profiling; CLINICAL RELEVANCE: Several of these entities have previously been implicated in rheumatoid arthritis and osteoarthritis of the knee, but many could potentially be used as novel biomarkers of PTAA. Most importantly, the findings suggest that metabolites could be used to distinguish synovial fluid from patients with PTAA.Copyright © The Author(s) 2014; CONCLUSION: This study identified inflammatory cytokines and metabolites present in the synovial fluid of PTAA; METHODS: Ankle joint synovial fluid was obtained from 20 patients with PTAA and 20 patients with no ankle pain and no radiographic evidence of ankle arthritis (control group). Synovial fluid samples were analyzed for IFN-gamma, TNF-alpha, MIP-1beta, MCP-1, IL-1beta, IL-1Ra, IL-4, IL-6, IL-8, IL-10, IL-13, and IL-15 using ELISA and for more than 3000 metabolites using liquid and gas chromatography with mass spectroscopy. To compare presence of cytokines and metabolites between groups, t tests were used. Random forest analysis was performed on metabolites to determine whether control and PTAA samples could be differentiated based on metabolic profile; RESULTS: IL-1Ra, IL-6, IL-8, IL-10, IL-15, and MCP-1 were significantly elevated in the PTAA group. In addition, 107 metabolites in the PTAA group were significantly altered, including derangement in amino acid, carbohydrate, lipid, and energy metabolism, extracellular matrix turnover, and collagen degradation. Random forest analysis yielded a predictive accuracy of 90% when using the metabolic profiles to distinguish between control and PTAA samples
UR  - http://dx.doi.org/10.1177/1071100714550652
ER  -