Apical Resection and Cryoinjury of Neonatal Mouse Heart.

MedStar author(s):
Citation: Methods in Molecular Biology. 2158:23-32, 2021.PMID: 32857362Institution: MedStar Franklin Square Medical CenterDepartment: SurgeryForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Cardiac Surgical Procedures/ae [Adverse Effects] | *Cryosurgery/ae [Adverse Effects] | *Heart Injuries/pa [Pathology] | *Heart/ph [Physiology] | *Regeneration | *Ventricular Remodeling | Animals | Animals, Newborn | Cell Proliferation | Female | Heart Injuries/et [Etiology] | Heart Injuries/rh [Rehabilitation] | Male | MiceYear: 2021ISSN:
  • 1064-3745
Name of journal: Methods in molecular biology (Clifton, N.J.)Abstract: Neonatal mouse hearts have a regenerative capacity similar to adult zebrafish. Different cardiac injury models have been established to investigate the regenerative capacity of neonatal mouse hearts, including ventricular amputation, cryoinjury, and ligation of a major coronary artery. While the ventricular resection model can be utilized to study how tissue forms and regenerates de novo, cryoinjury and coronary artery ligation are methods that might better mimic myocardial infarction by creating tissue damage and necrosis as opposed to the removal of healthy tissue in the ventricular amputation model. Here we describe methods of creating ventricular resection and cardiac cryoinjury in newborn mice.All authors: Darehzereshki A, Lien CL, Shen H, Sucov HMOriginally published: Methods in Molecular Biology. 2158:23-32, 2021.Fiscal year: FY2021Digital Object Identifier: Date added to catalog: 2020-10-06
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Journal Article MedStar Authors Catalog Article 32857362 Available 32857362

Neonatal mouse hearts have a regenerative capacity similar to adult zebrafish. Different cardiac injury models have been established to investigate the regenerative capacity of neonatal mouse hearts, including ventricular amputation, cryoinjury, and ligation of a major coronary artery. While the ventricular resection model can be utilized to study how tissue forms and regenerates de novo, cryoinjury and coronary artery ligation are methods that might better mimic myocardial infarction by creating tissue damage and necrosis as opposed to the removal of healthy tissue in the ventricular amputation model. Here we describe methods of creating ventricular resection and cardiac cryoinjury in newborn mice.

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