Intravenous administration of multipotent stromal cells and bone allograft modification to enhance allograft healing.

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Citation: Regenerative Medicine. 14(3):199-211, 2019 03.PMID: 30761943Institution: MedStar Union Memorial Hospital | MedStar Washington Hospital CenterDepartment: Orthobiologic Laboratory | Orthopaedic Surgery | PathologyForm of publication: Journal ArticleMedline article type(s): Journal ArticleSubject headings: *Bone Transplantation | *Mesenchymal Stem Cell Transplantation/mt [Methods] | *Mesenchymal Stem Cells/cy [Cytology] | *Multipotent Stem Cells/cy [Cytology] | *Osteogenesis | *Stromal Cells/cy [Cytology] | *Wound Healing | Administration, Intravenous | Allografts | Animals | Cells, Cultured | Humans | Mice | Mice, NudeYear: 2019ISSN:
  • 1746-0751
Name of journal: Regenerative medicineAbstract: AIM: This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs).CONCLUSION: Coating bone allograft with stromal cell-derived factor 1 and platelet-derived growth factor BB and intravenous injections of MSCs improved allograft incorporation.MATERIALS & METHODS: After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (Allo<sub>S-P</sub>). Allograft coated with copolymers alone (Allo), as controls, or Allo<sub>S-P</sub> was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3.RESULTS: At week 8, the total callus volume (both cartilaginous and bony callus) around the allograft was the largest in the Allo<sub>S-P</sub> + MSC group (p < 0.05).All authors: Lee M, Lee WH, Mitchell R, Paudel S, Schon L, Yang SY, Zahoor T, Zhang Z, Zhao HOriginally published: Regenerative Medicine. 2019 Feb 14Fiscal year: FY2019Digital Object Identifier: Date added to catalog: 2019-03-14
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Journal Article MedStar Authors Catalog Article 30761943 Available 30761943

AIM: This study investigated a coordinated strategy of revitalizing bone allograft with circulating multipotent stromal cells (MSCs).

CONCLUSION: Coating bone allograft with stromal cell-derived factor 1 and platelet-derived growth factor BB and intravenous injections of MSCs improved allograft incorporation.

MATERIALS & METHODS: After chemotactic and releasing assessments, stromal cell-derived factor 1 and platelet-derived growth factor BB in copolymers were coated on the bone allograft (Allo<sub>S-P</sub>). Allograft coated with copolymers alone (Allo), as controls, or Allo<sub>S-P</sub> was implanted into the femur of athymic mice, which received intravenous injections of human MSCs or saline at weeks 1, 2 and 3.

RESULTS: At week 8, the total callus volume (both cartilaginous and bony callus) around the allograft was the largest in the Allo<sub>S-P</sub> + MSC group (p < 0.05).

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