000			04275nam a22005777a 4500
008			160113s20152015 xxu||||| |||| 00| 0 eng d
022			_a1559-047X
040			_aOvid MEDLINE(R)
099			_a25162947
245			_aA multimodal assessment of melanin and melanocyte activity in abnormally pigmented hypertrophic scar.
251			_aJournal of Burn Care & Research. 36(1):77-86, 2015 Jan-Feb.
252			_aJ Burn Care Res. 36(1):77-86, 2015 Jan-Feb.
253			_aJournal of burn care & research : official publication of the American Burn Association
260			_c2015
260			_fFY2016
266			_d2016-01-13
501			_aAvailable online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present
520			_aUsing a validated swine model of human scar formation, hyperpigmented and hypopigmented scar samples were examined for their histological and optical properties to help elucidate the mechanisms and characteristics of dyspigmentation. Full-thickness wounds were created on the flanks of red Duroc pigs and allowed to heal. Biopsies from areas of hyperpigmentation, hypopigmentation, and uninjured tissue were fixed and embedded for histological examination using Azure B and primary antibodies to S100B, HMB45, and alpha-melanocyte-stimulating hormone (alpha-MSH). Spatial frequency domain imaging (SFDI) was then used to examine the optical properties of scars. Hyperpigmentation was first noticeable in healing wounds around weeks 2 to 3, gradually becoming darker. There was no significant difference in S100B staining for the presence of melanocytes between hyperpigmented and hypopigmented scar samples. Azure B staining of melanin was significantly greater in histological sections from hyperpigmented areas than in sections from both uninjured skin and hypopigmented scar (P < .0001). There was significantly greater staining for alpha-MSH in hyperpigmented samples compared with hypopigmented samples (P = .0121), and HMB45 staining was positive for melanocytes in hyperpigmented scar. SFDI at a wavelength of 632 nm resulted in an absorption coefficient map correlating with visibly hyperpigmented areas of scars. In a red Duroc model of hypertrophic scar formation, melanocyte number is similar in hyperpigmented and hypopigmented tissues. Hyperpigmented tissues, however, show a greater amount of melanin and alpha-MSH, along with immunohistochemical evidence of stimulated melanocytes. These observations encourage further investigation of melanocyte stimulation and the inflammatory environment within a wound that may influence melanocyte activity. Additionally, SFDI can be used to identify areas of melanin content in mature, pigmented scars, which may lead to its usefulness in wounds at earlier time points before markedly apparent pigmentation abnormalities.
546			_aEnglish
650			_a*Cicatrix, Hypertrophic/me [Metabolism]
650			_a*Cicatrix, Hypertrophic/pa [Pathology]
650			_a*Hyperpigmentation/me [Metabolism]
650			_a*Hypopigmentation/me [Metabolism]
650			_a*Melanins/me [Metabolism]
650			_a*Melanocytes/ph [Physiology]
650			_aalpha-MSH/me [Metabolism]
650			_aAnimals
650			_aCicatrix, Hypertrophic/et [Etiology]
650			_aDisease Models, Animal
650			_aHyperpigmentation/et [Etiology]
650			_aHyperpigmentation/pa [Pathology]
650			_aHypopigmentation/et [Etiology]
650			_aHypopigmentation/pa [Pathology]
650			_aMale
650			_aSwine
650			_aWound Healing/ph [Physiology]
651			_aMedStar Washington Hospital Center
656			_aSurgery/Burn Services
657			_aJournal Article
657			_aResearch Support, N.I.H., Extramural
657			_aResearch Support, Non-U.S. Gov't
700			_aJordan, Marion H
700			_aMoffatt, Lauren T
700			_aPaul, Dereck W
700			_aPrindeze, Nicholas J
700			_aShupp, Jeffrey W
700			_aTravis, Taryn E
790			_aGhassemi P, Jordan MH, Moffatt LT, Paul DW, Prindeze NJ, Ramella-Roman JC, Shupp JW, Travis TE
856			_uhttp://dx.doi.org/10.1097/BCR.0000000000000154 _zhttp://dx.doi.org/10.1097/BCR.0000000000000154
942			_cART _dArticle
999			_c1591 _d1591