MARC details
| 000 -LEADER |
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| fixed length control field |
03445nam a22003737a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
230411s2022022 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
2767-9764 |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
10.1158/2767-9764.CRC-22-0203 [doi] |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
CRC-22-0203 [pii] |
| 024 ## - OTHER STANDARD IDENTIFIER |
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| Standard number or code |
PMC10035501 [pmc] |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
36970725 |
| 245 ## - TITLE STATEMENT |
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| Title |
Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients. |
| 251 ## - Source |
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| Source |
Cancer Research Communications. 2(12):1617-1625, 2022 Dec. |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
Cancer Res Commun. 2(12):1617-1625, 2022 Dec. |
| 253 ## - Journal Name |
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| Journal name |
Cancer research communications |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Year |
2022 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Manufacturer |
FY2023 |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Publication date |
2022 Dec |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Publication status |
epublish |
| 265 ## - SOURCE FOR ACQUISITION/SUBSCRIPTION ADDRESS [OBSOLETE] |
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| Medline status |
PubMed-not-MEDLINE |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2023-04-11 |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
Prostate cancer is the most frequently diagnosed solid malignancy in men. African American (AA) men are at greater risk for developing prostate cancer, and experience higher mortality rates, as compared with Caucasian American men. However, mechanistic studies to understand this health disparity have been limited by the lack of relevant in vitro and in vivo models. There is an urgent need for preclinical cellular models to investigate molecular mechanisms underlying prostate cancer in AA men. We collected clinical specimens from radical prostatectomies of AA patients and established 10 paired tumor-derived and normal epithelial cell cultures from the same donors, which were further cultivated to extend the growth under "conditional reprogramming." Clinical and cellular annotations characterized these model cells as intermediate risk and predominantly diploid. Immunocytochemical analyses demonstrated variable expression levels of luminal (CK8) and basal (CK5, p63) markers in both normal and tumor cells. However, expression levels of TOPK, c-MYC, and N-MYC were markedly increased only in tumor cells. To determine cell utility for drug testing, we examined viability of cells following exposure to the antiandrogen (bicalutamide) and two PARP inhibitors (olaparib and niraparib) and observed decreased viability of tumor-derived cells as compared with viability of normal prostate-derived cells. |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
Significance: Cells derived from prostatectomies of AA patients conferred a bimodal cellular phenotype, recapitulating clinical prostate cellular complexity in this model cell system. Comparisons of viability responses of tumor derived to normal epithelial cells offer the potential for screening therapeutic drugs. Therefore, these paired prostate epithelial cell cultures provide an in vitro model system suitable for studies of molecular mechanisms in health disparities. Copyright © 2022 The Authors; Published by the American Association for Cancer Research. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
MedStar Georgetown University Hospital/MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Radiation Oncology Residency |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Carrasquilla, Michael |
| Institution Code |
MGUH |
| Program |
Radiation Oncology Residency |
| Degree |
MD |
| Resident year |
Resident PGY 5 |
| 790 ## - Authors |
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| All authors |
Jung M, Kowalczyk K, Hankins R, Bandi G, Kallakury B, Carrasquilla MA, Banerjee PP, Grindrod S, Dritschilo A |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="https://dx.doi.org/10.1158/2767-9764.CRC-22-0203">https://dx.doi.org/10.1158/2767-9764.CRC-22-0203</a> |
| Public note |
https://dx.doi.org/10.1158/2767-9764.CRC-22-0203 |
| 858 ## - ORCID |
|---|
| Orcid |
<a href="Carrasquilla, Michael A">Carrasquilla, Michael A</a> |
| ORCID text |
https://orcid.org/0000-0003-0957-0248 |
| Name |
Carrasquilla, Michael A |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Journal Article |
| Item type description |
Article |
