MARC details
| 000 -LEADER |
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| fixed length control field |
04581nam a22005897a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
141111s20142014 xxu||||| |||| 00| 0 eng d |
| 022 ## - INTERNATIONAL STANDARD SERIAL NUMBER |
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| International Standard Serial Number |
1559-047X |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
Ovid MEDLINE(R) |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
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| PMID |
24043235 |
| 245 ## - TITLE STATEMENT |
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| Title |
Regional neurovascular inflammation and apoptosis are detected after electrical contact injury. |
| 251 ## - Source |
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| Source |
Journal of Burn Care & Research. 35(1):11-20, 2014 Jan-Feb. |
| 252 ## - Abbreviated Source |
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| Abbreviated source |
J Burn Care Res. 35(1):11-20, 2014 Jan-Feb. |
| 253 ## - Journal Name |
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| Journal name |
Journal of burn care & research : official publication of the American Burn Association |
| 266 ## - Date added to catalog |
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| Date added to catalog |
2014-11-11 |
| 501 ## - WITH NOTE |
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| Local holdings |
Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present |
| 520 ## - SUMMARY, ETC. |
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| Abstract |
High-voltage electrical injuries are a devastating form of trauma often treated in burn centers. Examining superficial wounds alone may lead to an inaccurate assessment of local, regional, and systemic severity of injury. In this work, the neurovasculature at sites regionally distinct from the contact wound were assessed for cellular pathology. Nine male Sprague-Dawley rats subjected to 1000V direct-current shocks were separated into three groups: high-shock (>10-second contact), low-shock (<4-second contact), and control. Injury video was captured with a forward-looking infrared camera, and a thermal excitation analysis was performed. The neurovascular bundles from the iliofemoral region to the distal posterior tibial region were dissected from the hind limbs of the shocked animals and stained by immunohistochemistry for antibodies specific to apoptosis (APO) 1, caspase-3, activating transcription factor 3, high-mobility group box-1, granulocyte-macrophage colony-stimulating factor and interleukin-6. Real-time reverse-transcription polymerase chain reaction was used to quantify differential transcript levels of superoxide dismutases 1, 2, and 3 and heat-shock protein 70 from peripheral blood mononuclear cells and liver tissue. Finally, a protein array was used to identify key inflammatory cytokines in blood plasma. Significant dose-dependent trends were identified in apoptotic markers as well as inflammatory markers in both arterial and nerve tissues. Although arterial tissue exhibited a gradual decline in these markers proximally from the wound site, nerve tissue maintained a constant level at every location. Transcript analysis revealed an up-regulation of extracellular superoxide dismutase, and down-regulation of heat-shock protein 70, whereas plasma inflammatory cytokine levels indicated no significant 141111s. Thermal excitation analysis revealed a linear temperature increase, with a dose-dependent thermal maximum. In this study the authors have shown that neurovascular APO and inflammation are present at locations extremely proximal to electrical injury contact sites and this appears to be dose-dependent. Nerve tissue APO and inflammation may extend farther proximally than the iliofemoral region, and multiple proapoptotic mechanisms may be activated. No systemic inflammatory response was indicated in this study. |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Apoptosis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Burns, Electric/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Hindlimb/in [Injuries] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
*Inflammation/pa [Pathology] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Activating Transcription Factor 3/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Animals |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Biological Markers/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Burns, Electric/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Cytokines/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Granulocyte-Macrophage Colony-Stimulating Factor/me [Metabolism] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Hindlimb/bs [Blood Supply] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Hindlimb/ir [Innervation] |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Immunohistochemistry |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
In Situ Nick-End Labeling |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Male |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Photomicrography |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Rats |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Rats, Sprague-Dawley |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Real-Time Polymerase Chain Reaction |
| 651 ## - SUBJECT ADDED ENTRY--GEOGRAPHIC NAME |
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| Institution |
MedStar Washington Hospital Center |
| 656 ## - INDEX TERM--OCCUPATION |
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| Department |
Surgery/Burn Services |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Journal Article |
| 657 ## - INDEX TERM--FUNCTION |
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| Medline publication type |
Research Support, Non-U.S. Gov't |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Bullock, Rachel M |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Carney, Bonnie C |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Jo, Daniel Y |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Moffatt, Lauren T |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Ortiz, Rachel T T |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Paul, Dereck W |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Prindeze, Nicholas J |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Local Authors |
Shupp, Jeffrey W |
| 790 ## - Authors |
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| All authors |
Bullock RM, Carney BC, Jo DY, Moffatt LT, Ortiz RT, Paul DW, Prindeze NJ, Shupp JW |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| DOI |
<a href="http://dx.doi.org/10.1097/BCR.0b013e3182a2accd">http://dx.doi.org/10.1097/BCR.0b013e3182a2accd</a> |
| Public note |
http://dx.doi.org/10.1097/BCR.0b013e3182a2accd |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Koha item type |
Journal Article |
| Item type description |
Journal article |
