Mucosal expression of aquaporin 5 and epithelial barrier proteins in chronic rhinosinusitis with and without nasal polyps.

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Citation: Am J Otolaryngol. 35(3):377-83, 2014 May-Jun.American Journal of Otolaryngology. 35(3):377-83, 2014 May-Jun.PMID: 24612929Institution: MedStar Union Memorial HospitalDepartment: Otolaryngology-Head and Neck SurgeryForm of publication: Journal ArticleMedline article type(s): Journal Article | Research Support, Non-U.S. Gov'tSubject headings: *Aquaporin 5/an [Analysis] | *Membrane Proteins/an [Analysis] | *Nasal Mucosa/ch [Chemistry] | *Nasal Polyps/co [Complications] | *Rhinitis/me [Metabolism] | *Sinusitis/me [Metabolism] | Aquaporin 5/ph [Physiology] | Blotting, Western | Cadherins/an [Analysis] | Humans | Immunohistochemistry | Nasal Mucosa/pa [Pathology] | Prospective Studies | Real-Time Polymerase Chain Reaction | RNA/an [Analysis] | Septins/an [Analysis]Year: 2014ISSN:
  • 0196-0709
Name of journal: American journal of otolaryngologyAbstract: CONCLUSIONS: Collectively, these data suggest that the mucosal epithelial barrier is compromised in the context of CRS (predominantly in CRSwNP) when compared to control and that AQP5 acts as a key tight junction protein in the maintenance of mucosal water homeostasis. We hypothesize that AQP5 plays a possible role in the pathophysiology of mucosal edema and polyp formation.Copyright © 2014 Elsevier Inc. All rights reserved.METHODS: Mucosal membrane changes were evaluated through hematoxylin and eosin staining of the membrane barrier and immunohistochemical staining of AQP5 expression, a membrane channel protein that affects trans-epithelial water permeability and tissue edema. AQP5 expression was confirmed by real-time PCR (rt-PCR) and western blot. Levels of other membrane proteins, including E-cadherin and Septin-2, were also assessed.OBJECTIVES: The purpose of this study is to characterize the association between altered epithelial barrier function, represented by changes in histology and differential expression of the mucosal water membrane permeability protein aquaporin 5 (AQP5), and the pathophysiology of chronic refractory sinusitis (CRS) in patients with and without nasal polyposis.PARTICIPANTS: Sinonasal samples were obtained from seven CRS subjects with nasal polyps (CRSwNP), seven CRS without nasal polyposis (CRSsNP), and five control healthy patients.RESULTS: CRSwNP patients showed substantial histologic evidence of membrane remodeling with increased edema and glandular hyperplasia. The epithelial expression of AQP5 was significantly lower in CRSwNP as compared to CRSsNP or control. There was no significant difference in the expression of E-cadherin and Septin-2.SETTING: Tertiary rhinology referral center.STUDY DESIGN: Prospective clinical study.All authors: Alqudah MA, Basaraba RJ, Cope E, Kirk N, Leid JG, Shikani AH, Shikani HJ, Sidhaye VKFiscal year: FY2014Digital Object Identifier: Date added to catalog: 2016-07-15
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Journal Article MedStar Authors Catalog Article 24612929 Available 24612929

CONCLUSIONS: Collectively, these data suggest that the mucosal epithelial barrier is compromised in the context of CRS (predominantly in CRSwNP) when compared to control and that AQP5 acts as a key tight junction protein in the maintenance of mucosal water homeostasis. We hypothesize that AQP5 plays a possible role in the pathophysiology of mucosal edema and polyp formation.Copyright © 2014 Elsevier Inc. All rights reserved.

METHODS: Mucosal membrane changes were evaluated through hematoxylin and eosin staining of the membrane barrier and immunohistochemical staining of AQP5 expression, a membrane channel protein that affects trans-epithelial water permeability and tissue edema. AQP5 expression was confirmed by real-time PCR (rt-PCR) and western blot. Levels of other membrane proteins, including E-cadherin and Septin-2, were also assessed.

OBJECTIVES: The purpose of this study is to characterize the association between altered epithelial barrier function, represented by changes in histology and differential expression of the mucosal water membrane permeability protein aquaporin 5 (AQP5), and the pathophysiology of chronic refractory sinusitis (CRS) in patients with and without nasal polyposis.

PARTICIPANTS: Sinonasal samples were obtained from seven CRS subjects with nasal polyps (CRSwNP), seven CRS without nasal polyposis (CRSsNP), and five control healthy patients.

RESULTS: CRSwNP patients showed substantial histologic evidence of membrane remodeling with increased edema and glandular hyperplasia. The epithelial expression of AQP5 was significantly lower in CRSwNP as compared to CRSsNP or control. There was no significant difference in the expression of E-cadherin and Septin-2.

SETTING: Tertiary rhinology referral center.

STUDY DESIGN: Prospective clinical study.

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