Cutaneous Thermal Injury Modulates Blood and Skin Metabolomes Differently in a Murine Model.
Cutaneous Thermal Injury Modulates Blood and Skin Metabolomes Differently in a Murine Model.
- 2021
Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present
As the field of metabolomics develops further, investigations of how the metabolome is affected following thermal injury may be helpful to inform diagnostics and guide treatments. In this study changes to the metabolome were tested and validated in a murine burn injury model. After a 30% total body surface scald injury or sham procedure sera and skin biopsies were collected at 1, 2, 6, or 24 hours. Burn-specific changes in the metabolome were detected compared to sham animals. The sera metabolome exhibited a more rapid response to burn injury than that of the skin and it peaked more proximal to injury (6 vs 24 hours). Progression of metabolic response in the skin was less synchronous and showed a higher overlap of the significantly modified metabolites (SMMs) among tested time-points. Top affected pathways identified by SMMs of skin included inositol phosphate metabolism, ascorbate and alderate metabolism, caffeine metabolism, and the pentose phosphate pathway. Future research is warranted in human and larger animal models to further elucidate the role of metabolomic perturbations and the pathophysiology following burn injury. Copyright (c) The Author(s) 2020. Published by Oxford University Press on behalf of the American Burn Association.
English
1559-047X
10.1093/jbcr/iraa209 [doi] 6029941 [pii]
*Biomarkers/me [Metabolism]
*Burns/me [Metabolism]
*Hot Temperature
*Metabolome
Animals
Burns/pa [Pathology]
Disease Models, Animal
Metabolomics/mt [Methods]
Mice
MedStar Health Research Institute
MedStar Washington Hospital Center
Firefighters' Burn and Surgical Research Laboratory
Surgery/Burn Services
Journal Article
Available online through MWHC library: 2006 - present, Available in print through MWHC library: 2006 - present
As the field of metabolomics develops further, investigations of how the metabolome is affected following thermal injury may be helpful to inform diagnostics and guide treatments. In this study changes to the metabolome were tested and validated in a murine burn injury model. After a 30% total body surface scald injury or sham procedure sera and skin biopsies were collected at 1, 2, 6, or 24 hours. Burn-specific changes in the metabolome were detected compared to sham animals. The sera metabolome exhibited a more rapid response to burn injury than that of the skin and it peaked more proximal to injury (6 vs 24 hours). Progression of metabolic response in the skin was less synchronous and showed a higher overlap of the significantly modified metabolites (SMMs) among tested time-points. Top affected pathways identified by SMMs of skin included inositol phosphate metabolism, ascorbate and alderate metabolism, caffeine metabolism, and the pentose phosphate pathway. Future research is warranted in human and larger animal models to further elucidate the role of metabolomic perturbations and the pathophysiology following burn injury. Copyright (c) The Author(s) 2020. Published by Oxford University Press on behalf of the American Burn Association.
English
1559-047X
10.1093/jbcr/iraa209 [doi] 6029941 [pii]
*Biomarkers/me [Metabolism]
*Burns/me [Metabolism]
*Hot Temperature
*Metabolome
Animals
Burns/pa [Pathology]
Disease Models, Animal
Metabolomics/mt [Methods]
Mice
MedStar Health Research Institute
MedStar Washington Hospital Center
Firefighters' Burn and Surgical Research Laboratory
Surgery/Burn Services
Journal Article